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题名: Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail
作者: Hu, Ying1, 2;  Luan, Hongwei1, 2;  Ge, Guangbo1, 2;  Liu, Huixin1, 2;  Zhang, Yanyan1, 2;  Zhou, Kun1, 2;  Liu, Yong1;  Yang, Ling1
关键词: beta-D-Glycosidase ;  Transglycosylation activity ;  Kinetics ;  Allosteric effect ;  The China white jade snail
刊名: JOURNAL OF BIOTECHNOLOGY
发表日期: 2009-02-05
DOI: 10.1016/j.jbiotec.2008.12.006
卷: 139, 期:3, 页:229-235
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A beta P-D-glycosidase (G1)from the China white Jade snail showed non-Michaelis-Menten mode in catalyzing the reaction using pNPGlu and pNPFuc as the substrate and monitoring the released pNP. We determined quantitatively both the transglycosidic and hydrolytic products of pNPGlu and pNPFuc solvolysis for the detailed kinetic analysis on G1-catalyzed hydrolysis and transglycosylation reaction. The inhibition kinetic studies using deoxynojirimycin (DNJ) and butanol as inhibitors were preceded. DNJ only inhibited competitively the hydrolysis of cellobiose and pNPGlu while "activated" the transglycosylation of pNPGlu and pNPFuc. This was evident from the increased V(max)tr value with no change of the apparent K(m)tr. In contrast, butanol exhibited a competitive inhibition to the transglycosylation reaction and non-competitive inhibition to the hydrolysis. The results indicated that the non-Michaelis-Menten kinetic behavior was caused by the co-occurrence of substrate transglycosylation reaction. This study provided a simple method to increase the transglycosylation yield by using DNJ to inhibit hydrolysis. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: THERMOSTABLE BETA-GLYCOSIDASE ;  D-GLUCOSIDASE ;  ENZYMATIC CHARACTERIZATION ;  OLIGOSACCHARIDE SYNTHESIS ;  THERMOTOGA-NEAPOLITANA ;  SUBSTRATE-SPECIFICITY ;  THERMUS-THERMOPHILUS ;  GLYCOSYL HYDROLASES ;  PYROCOCCUS-FURIOSUS ;  ASPERGILLUS-NIGER
语种: 英语
WOS记录号: WOS:000263742300006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141569
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Hu, Ying,Luan, Hongwei,Ge, Guangbo,et al. Deoxynojirimycin enhanced the transglycosylation activity of a glycosidase from the China white jade snail[J]. JOURNAL OF BIOTECHNOLOGY,2009,139(3):229-235.
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