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A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix
Liu, Tingjiao1,2; Li, Chunyu1; Li, Hongjing3; Zeng, Shaojiang1; Qin, Jianhua1; Lin, Bingcheng1
关键词Invasion Microfluidic 3-d
刊名ELECTROPHORESIS
2009-12-01
DOI10.1002/elps.200900289
30期:24页:4285-4291
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]EPIDERMAL-GROWTH-FACTOR ; EXTRACELLULAR-MATRIX ; CULTURE SYSTEM ; MIGRATION ; INVADOPODIA ; CHEMOTAXIS ; DEGRADATION ; PROTEOLYSIS ; METASTASIS ; HYDROGEL
英文摘要A microfluidic device was developed for the study of directed invasion of cancer cells in 3-D matrix with concentration gradient. This device consists of two parallel perfusion channels connected by two cell culture chambers. To mimic extracellular matrix (ECM), gelled basement membrane extract (BME) was used to support 3-D distribution of breast cancer cells (MCF7) in cell culture chambers. A stable linear concentration gradient of epidermal growth factor (EGF) was generated across the chambers by continuous perfusion. Using the device, we investigated MCF7 cell invasion induced by different concentrations of EGF in 3-D matrix. It was found that cancer cells responded to EGF stimulation with forming cellular protrusions and migrating towards high EGF concentration. We further investigated the anti-invasion effect of GM 6001, a matrix metalloproteinase inhibitor. We identified that matrix metalloproteinase inhibition repressed both cellular protrusion formation and cell migration in 3-D matrix. These findings suggest that EGF is able to induce MCF7 cell invasion in 3-D extracellular matrix and this effect is dependent on proteolytic activity. This device is relatively simple to construct and operate. It should be a useful platform for elucidating the mechanism of cancer invasion and screening anti-invasion drugs for cancer therapy.
语种英语
WOS记录号WOS:000273187800019
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141586
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Sect Oral Pathol, Coll Stomatol, Dalian, Peoples R China
3.Dalian Med Univ, Dept Orthoped, Affiliated Hosp 1, Dalian, Peoples R China
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Liu, Tingjiao,Li, Chunyu,Li, Hongjing,et al. A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix[J]. ELECTROPHORESIS,2009,30(24):4285-4291.
APA Liu, Tingjiao,Li, Chunyu,Li, Hongjing,Zeng, Shaojiang,Qin, Jianhua,&Lin, Bingcheng.(2009).A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix.ELECTROPHORESIS,30(24),4285-4291.
MLA Liu, Tingjiao,et al."A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix".ELECTROPHORESIS 30.24(2009):4285-4291.
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