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题名: A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix
作者: Liu, Tingjiao1, 2;  Li, Chunyu1;  Li, Hongjing3;  Zeng, Shaojiang1;  Qin, Jianhua1;  Lin, Bingcheng1
关键词: Invasion ;  Microfluidic ;  3-D
刊名: ELECTROPHORESIS
发表日期: 2009-12-01
DOI: 10.1002/elps.200900289
卷: 30, 期:24, 页:4285-4291
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A microfluidic device was developed for the study of directed invasion of cancer cells in 3-D matrix with concentration gradient. This device consists of two parallel perfusion channels connected by two cell culture chambers. To mimic extracellular matrix (ECM), gelled basement membrane extract (BME) was used to support 3-D distribution of breast cancer cells (MCF7) in cell culture chambers. A stable linear concentration gradient of epidermal growth factor (EGF) was generated across the chambers by continuous perfusion. Using the device, we investigated MCF7 cell invasion induced by different concentrations of EGF in 3-D matrix. It was found that cancer cells responded to EGF stimulation with forming cellular protrusions and migrating towards high EGF concentration. We further investigated the anti-invasion effect of GM 6001, a matrix metalloproteinase inhibitor. We identified that matrix metalloproteinase inhibition repressed both cellular protrusion formation and cell migration in 3-D matrix. These findings suggest that EGF is able to induce MCF7 cell invasion in 3-D extracellular matrix and this effect is dependent on proteolytic activity. This device is relatively simple to construct and operate. It should be a useful platform for elucidating the mechanism of cancer invasion and screening anti-invasion drugs for cancer therapy.
关键词[WOS]: EPIDERMAL-GROWTH-FACTOR ;  EXTRACELLULAR-MATRIX ;  CULTURE SYSTEM ;  MIGRATION ;  INVADOPODIA ;  CHEMOTAXIS ;  DEGRADATION ;  PROTEOLYSIS ;  METASTASIS ;  HYDROGEL
语种: 英语
WOS记录号: WOS:000273187800019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141586
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Sect Oral Pathol, Coll Stomatol, Dalian, Peoples R China
3.Dalian Med Univ, Dept Orthoped, Affiliated Hosp 1, Dalian, Peoples R China

Recommended Citation:
Liu, Tingjiao,Li, Chunyu,Li, Hongjing,et al. A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix[J]. ELECTROPHORESIS,2009,30(24):4285-4291.
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