DICP OpenIR
A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix
Liu, Tingjiao1,2; Li, Chunyu1; Li, Hongjing3; Zeng, Shaojiang1; Qin, Jianhua1; Lin, Bingcheng1
KeywordInvasion Microfluidic 3-d
Source PublicationELECTROPHORESIS
2009-12-01
DOI10.1002/elps.200900289
Volume30Issue:24Pages:4285-4291
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordEPIDERMAL-GROWTH-FACTOR ; EXTRACELLULAR-MATRIX ; CULTURE SYSTEM ; MIGRATION ; INVADOPODIA ; CHEMOTAXIS ; DEGRADATION ; PROTEOLYSIS ; METASTASIS ; HYDROGEL
AbstractA microfluidic device was developed for the study of directed invasion of cancer cells in 3-D matrix with concentration gradient. This device consists of two parallel perfusion channels connected by two cell culture chambers. To mimic extracellular matrix (ECM), gelled basement membrane extract (BME) was used to support 3-D distribution of breast cancer cells (MCF7) in cell culture chambers. A stable linear concentration gradient of epidermal growth factor (EGF) was generated across the chambers by continuous perfusion. Using the device, we investigated MCF7 cell invasion induced by different concentrations of EGF in 3-D matrix. It was found that cancer cells responded to EGF stimulation with forming cellular protrusions and migrating towards high EGF concentration. We further investigated the anti-invasion effect of GM 6001, a matrix metalloproteinase inhibitor. We identified that matrix metalloproteinase inhibition repressed both cellular protrusion formation and cell migration in 3-D matrix. These findings suggest that EGF is able to induce MCF7 cell invasion in 3-D extracellular matrix and this effect is dependent on proteolytic activity. This device is relatively simple to construct and operate. It should be a useful platform for elucidating the mechanism of cancer invasion and screening anti-invasion drugs for cancer therapy.
Language英语
WOS IDWOS:000273187800019
Citation statistics
Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141586
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Dalian Med Univ, Sect Oral Pathol, Coll Stomatol, Dalian, Peoples R China
3.Dalian Med Univ, Dept Orthoped, Affiliated Hosp 1, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Liu, Tingjiao,Li, Chunyu,Li, Hongjing,et al. A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix[J]. ELECTROPHORESIS,2009,30(24):4285-4291.
APA Liu, Tingjiao,Li, Chunyu,Li, Hongjing,Zeng, Shaojiang,Qin, Jianhua,&Lin, Bingcheng.(2009).A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix.ELECTROPHORESIS,30(24),4285-4291.
MLA Liu, Tingjiao,et al."A microfluidic device for characterizing the invasion of cancer cells in 3-D matrix".ELECTROPHORESIS 30.24(2009):4285-4291.
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