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题名: Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel
作者: Li, Sheng-Hua1;  Wang, Jun1;  Zhao, Mei-Ping1
关键词: BSA-Cu(II) complex ;  Coordinate interaction ;  Molecularly imprinted hydrogel
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2009-10-01
DOI: 10.1002/jssc.200900250
卷: 32, 期:19, 页:3359-3363
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A novel molecularly imprinted hydrogel for bovine serum albumin (BSA) was prepared using cupric ion as the bridge between the template BSA and the functional monomer 4-vinylpyridine. N-Isopropylacrylamide (NIPA) was used as an assistant monomer to provide the stimuli-responsibility of the polymer. The adsorption conditions of BSA on the BSA-Cu(II)-imprinted hydrogel were optimized considering the influences of pH, temperature, and salt concentration. The proteins bound on the imprinted hydrogel can be easily recovered under mild conditions by using 10 mmol/L ethylene diamine tetraacetic acid (EDTA) (pH 7.0) containing 150 mmol/L NaCl as the eluting solution. The imprinting effect and adsorption capacity of the polymer were found to be significantly improved compared to the hydrogel prepared in the absence of cupric ion. The results demonstrated the advantages of using a template-metal ion-monomer coordination system to strengthen the interaction between the protein and monomer. The effects of different metals ions including Zn(II), Ni(II), Co(II), Cd(II), and Al(III) on the recognition ability of the BSA-Cu(II)-imprinted hydrogel were also investigated. The polymer showed high selectivity toward both the template protein and the cupric ion.
关键词[WOS]: ELECTROSTATIC FUNCTIONAL-GROUPS ;  POLYACRYLAMIDE-GEL BEADS ;  PROTEIN RECOGNITION ;  BINDING ;  HEMOGLOBIN ;  HISTIDINE ;  POLYMERS ;  SURFACES ;  SITE
语种: 英语
WOS记录号: WOS:000270964800014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141603
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China

Recommended Citation:
Li, Sheng-Hua,Wang, Jun,Zhao, Mei-Ping. Cupric ion enhanced molecular imprinting of bovine serum albumin in hydrogel[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(19):3359-3363.
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