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题名: Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry
作者: Wang, Jiangshan1, 2;  van der Heijden, Rob1;  Spijksma, Gerwin1;  Reijmers, Theo1;  Wang, Mei3;  Xu, Guowang2;  Hankemeier, Thomas1;  van der Greef, Jan1, 3
关键词: Alkaloid profiling ;  Aconitum carmichaeli Debx ;  Herbal medicine ;  LC-MS ;  MALDI-MS
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2009-03-13
DOI: 10.1016/j.chroma.2008.11.077
卷: 1216, 期:11, 页:2169-2178
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) method was developed for the high throughput and robust qualitative profiling of alkaloids in Fuzi-the processed lateral roots of the Chinese herbal medicine Aconitum carmichaeli Debx (A. carmichaeli). After optimization, powdered roots - without any further sample preparation - Could be Used to screen for the presence or Aconitum alkaloids. Furthermore, the semi-quantitative potential of MALDI-MS was confirmed using liquid chromatography-mass spectrometry (LC-MS) as reference. In total over sixty alkaloids were detected by LC-MS and fifteen of them were tentatively identified. Both MALDI-MS and LC-MS analysis revealed significant variation in alkaloid content in different (commercial) samples. LC-MS analysis of three toxic alkaloids in 14 batches of Fuzi resulted in a variation of their concentrations expressed as RSDs of 138%, 99% and 221% for aconitine, hypaconitine and mesaconitine, respectively. The variation in concentrations (expressed as RSD) of about the ninety constituents detected were classified as follows: 13 constituents showed an RSD of 77-100%, 46 with an RSD of 100-150%, 21 with an RSD of 150-200% and 9 Constituents with an RSD in concentration of 200-235%. These results demonstrate a strong difference in chemical composition of the various Fuzi and illustrate the necessity of adequate QA/QC procedures for both safety and efficiency of herbal medicine. The described analytical procedures for alkaloid profiling could play a role in these procedures. (C) 2008 Elsevier B.V. All rights reserved.
关键词[WOS]: ACONITUM ALKALOIDS ;  CAPILLARY-ELECTROPHORESIS ;  QUANTITATIVE-DETERMINATION ;  URINE SAMPLES ;  BODY-FLUIDS ;  SEPARATION ;  ROOTS ;  SPECTROSCOPY ;  TUBERS ;  TISSUE
语种: 英语
WOS记录号: WOS:000264226500026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141613
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Leiden Univ, Leiden Amsterdam Ctr Drug Res, Div Analyt Biosci, NL-2300 RA Leiden, Netherlands
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.TNO, Qual Life & SU BioMed, NL-3700 AJ Zeist, Netherlands

Recommended Citation:
Wang, Jiangshan,van der Heijden, Rob,Spijksma, Gerwin,et al. Alkaloid profiling of the Chinese herbal medicine Fuzi by combination of matrix-assisted laser desorption ionization mass spectrometry with liquid chromatography-mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(11):2169-2178.
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