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题名: Determination of Arachidonic Acid and Its Endogenous Eicosanoid Metabolites in Mice Tissues Using Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry
作者: Gu Yan1, 2;  Shi Xian-Zhe1;  Zhao Su-Min1;  Gu Qun1;  Lu Xin1;  Xu Guo-Wang1
关键词: Liquid chromatography-tandem mass spectrometry ;  Arachidonic acid ;  Tissue
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2010-08-01
DOI: 10.3724/SP.J.1096.2010.01089
卷: 38, 期:8, 页:1089-1094
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A reversed phase liquid chromatography/tandem mass spectrometry method was developed to simultaneously analyze arachidonic acid(AA) and its metabolites such as prostaglandins(PGs), thromboxane (TXB(2)), epoxyeicosatrienoic acids(EETs), dihydroxyeicosatrienoic acids(DHETs) and hydroxyeicosatetraenoic acids(HETEs) through the corresponding cyclooxygenase, cytochrome P450, and lipoxygenase pathways in mice tissues. Ethyl acetate extraction was employed for sample preparation after sonication in an ice bath, and the organic phase was dried under vacuum and dissolved in methanol for the LC-MS/MS analysis. 22 standard compounds were separated within 50 min using a gradient elution of acetonitrile and water with 0.05% formic acid (V/V) on a C(18) column (250 mm x 4.6 mm, 5 mu m). PGB(1) and deuterium labeled 15-HETE-d8 and AA-d8 were used as internal standards. Mass spectrometric detection was performed using negative electrospray ionization and multiple reaction monitoring. The method was validated and shown to be accurate(recovery values 70.7% - 123.0%), precise(RSD < 20%) and sensitive(LOQ 3 - 250 mu g/L). The method was applied to separate arachidonic acid(AA) and its metabolites in lung, liver and spleen tissues in mice and quantify eicosanoid levels in lung tissue. The results were 11-HETE (58.3 +/- 11.6) ng/g, 15-HETE (52.6 +/- 15.5) ng/g, PGE(2)(210 +/- 102) ng/g, PGD(2) (20.3 +/- 13.9) ng/g, PGJ(2)(39.2 +/- 15.9) ng/g, TXB(2) (58.3 +/- 28.4) ng/g, PGF(2 alpha)(3.8 +/- 6.0) ng/g and AA(1980 +/- 544) ng/g, respectively.
关键词[WOS]: PROSTAGLANDINS ;  THROMBOXANE ;  VALIDATION
语种: 英语
WOS记录号: WOS:000281530100002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141629
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Shenyang Pharmaceut Univ, Sch Pharm, Dept Pharmaceut Anal, Shenyang 110016, Peoples R China

Recommended Citation:
Gu Yan,Shi Xian-Zhe,Zhao Su-Min,et al. Determination of Arachidonic Acid and Its Endogenous Eicosanoid Metabolites in Mice Tissues Using Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(8):1089-1094.
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