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题名: Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry
作者: Wang, Wenzhao2;  Feng, Bo1;  Li, Xiang2;  Yin, Peiyuan2;  Gao, Peng2;  Zhao, Xinjie2;  Lu, Xin2;  Zheng, Minhua1;  Xu, Guowang2
刊名: MOLECULAR BIOSYSTEMS
发表日期: 2010
DOI: 10.1039/c004994h
卷: 6, 期:10, 页:1947-1955
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Colorectal carcinoma (CRC) is the third most commonly encountered cancer and fourth cause of cancer-associated death worldwide. Abundant studies have demonstrated that one of the best effective therapies for enhancing the 5-year survival rate of patients is to diagnose the disease at an early stage. Urine metabonomics is widely being utilized as an efficient platform to investigate the metabolic changes and discover the potential biomarkers of malignant diseases. In this study both ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and online affinity solid phase extraction-high performance liquid chromatography (SPE-HPLC) were used to analyze the urinary metabolites from 34 healthy volunteers, 34 benign colorectal tumor and 50 colorectal carcinoma patients to produce comprehensive metabolic profiling data. A reliable separation between the control and disease groups as well as significantly changed metabolites were obtained from orthogonal signal correction partial least squares models which were built based on the two separate data sets from UPLC-MS and affinity SPE-HPLC, respectively. 15 metabolites, showing the metabolic disorders of CRC, were identified finally. These metabolites were found to be related to glutamine metabolism, fatty acid oxidation, nucleotide biosynthesis and protein metabolism.
关键词[WOS]: CANCER-PATIENTS ;  MODIFIED NUCLEOSIDES ;  TUMOR-MARKERS ;  FATTY-ACID ;  METABONOMICS ;  DIAGNOSIS ;  THERAPY ;  COLON ;  MS ;  IDENTIFICATION
语种: 英语
WOS记录号: WOS:000281772300028
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141631
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Surg, Shanghai 200025, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Wang, Wenzhao,Feng, Bo,Li, Xiang,et al. Urinary metabolic profiling of colorectal carcinoma based on online affinity solid phase extraction-high performance liquid chromatography and ultra performance liquid chromatography-mass spectrometry[J]. MOLECULAR BIOSYSTEMS,2010,6(10):1947-1955.
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