DICP OpenIR
A Fully Automated System with Online Sample Loading, Isotope Dimethyl Labeling and Multidimensional Separation for High-Throughput Quantitative Proteome Analysis
Wang, Fangjun1; Chen, Rui1; Zhu, Jun1; Sun, Deguang2; Song, Chunxia1; Wu, Yifeng2; Ye, Mingliang1; Wang, Liming2; Zou, Hanfa1
刊名ANALYTICAL CHEMISTRY
2010-04-01
DOI10.1021/ac100075y
82期:7页:3007-3015
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]DIFFERENCE GEL-ELECTROPHORESIS ; SPECTROMETRY-BASED PROTEOMICS ; CODED AFFINITY TAGS ; HEPATOCELLULAR-CARCINOMA ; MASS-SPECTROMETRY ; BIOMARKER DISCOVERY ; CELL-CULTURE ; AMINO-ACIDS ; PROTEINS ; IDENTIFICATION
英文摘要Multidimensional separation is often applied for large-scale qualitative and quantitative proteome analysis. A fully automated system with integration of a reversed phase-strong cation exchange (RP-SCX) biphasic trap column into vented sample injection system was developed to realize online sample loading, isotope dimethyl labeling and online multidimensional separation of the proteome samples. Comparing to conventionally manual isotope labeling and off-line fractionation technologies, this system is fully automated and time-saving, which is benefit for improving the quantification reproducibility and accuracy. As phosphate SCX monolith was integrated into the biphasic trap column, high sample injection flow rate and high-resolution stepwise fractionation could be easily achieved. similar to 1000 proteins could be quantified in similar to 30 h proteome analysis, and the proteome coverage of quantitative analysis can be further greatly improved by prolong the multidimensional separation time. This system was applied to analyze the different protein expression level of HCC and normal human liver tissues. After three times replicated analysis, finally 94 up-regulated and 249 down-regulated (HCC/Normal) proteins were successfully obtained. These significantly regulated proteins are widely validated by both gene and proteins expression studies previously. Such as some enzymes involved in urea cycle, methylation cycle and fatty acids catabolism in liver were all observed down-regulated.
语种英语
WOS记录号WOS:000276004000056
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141637
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 1, Dalian 116027, Peoples R China
推荐引用方式
GB/T 7714
Wang, Fangjun,Chen, Rui,Zhu, Jun,et al. A Fully Automated System with Online Sample Loading, Isotope Dimethyl Labeling and Multidimensional Separation for High-Throughput Quantitative Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2010,82(7):3007-3015.
APA Wang, Fangjun.,Chen, Rui.,Zhu, Jun.,Sun, Deguang.,Song, Chunxia.,...&Zou, Hanfa.(2010).A Fully Automated System with Online Sample Loading, Isotope Dimethyl Labeling and Multidimensional Separation for High-Throughput Quantitative Proteome Analysis.ANALYTICAL CHEMISTRY,82(7),3007-3015.
MLA Wang, Fangjun,et al."A Fully Automated System with Online Sample Loading, Isotope Dimethyl Labeling and Multidimensional Separation for High-Throughput Quantitative Proteome Analysis".ANALYTICAL CHEMISTRY 82.7(2010):3007-3015.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Fangjun]的文章
[Chen, Rui]的文章
[Zhu, Jun]的文章
百度学术
百度学术中相似的文章
[Wang, Fangjun]的文章
[Chen, Rui]的文章
[Zhu, Jun]的文章
必应学术
必应学术中相似的文章
[Wang, Fangjun]的文章
[Chen, Rui]的文章
[Zhu, Jun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。