中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation
作者: Wang Ena1;  Duan Shujing1;  He Xiwen1;  Chen Langxing1;  Li Wenyou1;  Zhang Yukui1, 2
关键词: silica ;  strong anion-exchange chromatography ;  atom transfer radical polymerization ;  protein's separation
刊名: ACTA CHIMICA SINICA
发表日期: 2010-12-14
卷: 68, 期:23, 页:2421-2428
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A new strong anion-exchange stationary phase for HPLC has been synthesized by surface-initiated atom transfer radical polymerization (ATRP). Firstly, 2-bromoisobutyryl bromide as the ATRP initiator was attached onto the macroporous silica via chemical reaction. In the next stage, methacrylatoethyl trimethyl ammonium chloride (MATA) carrying strong anion exchanger groups as the monomer was polymerized on the initiator-immobilized particles via surface-initiated ATRP. After studying the adsorption properties, the suitable support was packed into stainless steel column to separate acid proteins by the linear gradient elution in 20 min at flow rate of 0.75 mL/min. The effects of flow rate, pH and organic solvents on the separation and retention ability of proteins were studied in detail. This support was also used for the separation and purification of ovalbumin from egg in only one step.
关键词[WOS]: STATIONARY-PHASE ;  SURFACE ;  SILICA ;  BRUSHES ;  NANOPARTICLES ;  FABRICATION
语种: 英语
WOS记录号: WOS:000286082500008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141654
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Wang Ena,Duan Shujing,He Xiwen,et al. Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation[J]. ACTA CHIMICA SINICA,2010,68(23):2421-2428.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Wang Ena]'s Articles
 [Duan Shujing]'s Articles
 [He Xiwen]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Wang Ena]‘s Articles
 [Duan Shujing]‘s Articles
 [He Xiwen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace