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Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation
Wang Ena1; Duan Shujing1; He Xiwen1; Chen Langxing1; Li Wenyou1; Zhang Yukui1,2
关键词Silica Strong Anion-exchange Chromatography Atom Transfer Radical Polymerization Protein's Separation
刊名ACTA CHIMICA SINICA
2010-12-14
68期:23页:2421-2428
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]STATIONARY-PHASE ; SURFACE ; SILICA ; BRUSHES ; NANOPARTICLES ; FABRICATION
英文摘要A new strong anion-exchange stationary phase for HPLC has been synthesized by surface-initiated atom transfer radical polymerization (ATRP). Firstly, 2-bromoisobutyryl bromide as the ATRP initiator was attached onto the macroporous silica via chemical reaction. In the next stage, methacrylatoethyl trimethyl ammonium chloride (MATA) carrying strong anion exchanger groups as the monomer was polymerized on the initiator-immobilized particles via surface-initiated ATRP. After studying the adsorption properties, the suitable support was packed into stainless steel column to separate acid proteins by the linear gradient elution in 20 min at flow rate of 0.75 mL/min. The effects of flow rate, pH and organic solvents on the separation and retention ability of proteins were studied in detail. This support was also used for the separation and purification of ovalbumin from egg in only one step.
语种英语
WOS记录号WOS:000286082500008
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141654
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
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GB/T 7714
Wang Ena,Duan Shujing,He Xiwen,et al. Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation[J]. ACTA CHIMICA SINICA,2010,68(23):2421-2428.
APA Wang Ena,Duan Shujing,He Xiwen,Chen Langxing,Li Wenyou,&Zhang Yukui.(2010).Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation.ACTA CHIMICA SINICA,68(23),2421-2428.
MLA Wang Ena,et al."Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation".ACTA CHIMICA SINICA 68.23(2010):2421-2428.
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