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Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation
Wang Ena1; Duan Shujing1; He Xiwen1; Chen Langxing1; Li Wenyou1; Zhang Yukui1,2
KeywordSilica Strong Anion-exchange Chromatography Atom Transfer Radical Polymerization Protein's Separation
Source PublicationACTA CHIMICA SINICA
2010-12-14
Volume68Issue:23Pages:2421-2428
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
WOS KeywordSTATIONARY-PHASE ; SURFACE ; SILICA ; BRUSHES ; NANOPARTICLES ; FABRICATION
AbstractA new strong anion-exchange stationary phase for HPLC has been synthesized by surface-initiated atom transfer radical polymerization (ATRP). Firstly, 2-bromoisobutyryl bromide as the ATRP initiator was attached onto the macroporous silica via chemical reaction. In the next stage, methacrylatoethyl trimethyl ammonium chloride (MATA) carrying strong anion exchanger groups as the monomer was polymerized on the initiator-immobilized particles via surface-initiated ATRP. After studying the adsorption properties, the suitable support was packed into stainless steel column to separate acid proteins by the linear gradient elution in 20 min at flow rate of 0.75 mL/min. The effects of flow rate, pH and organic solvents on the separation and retention ability of proteins were studied in detail. This support was also used for the separation and purification of ovalbumin from egg in only one step.
Language英语
WOS IDWOS:000286082500008
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141654
Collection中国科学院大连化学物理研究所
Affiliation1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China
Recommended Citation
GB/T 7714
Wang Ena,Duan Shujing,He Xiwen,et al. Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation[J]. ACTA CHIMICA SINICA,2010,68(23):2421-2428.
APA Wang Ena,Duan Shujing,He Xiwen,Chen Langxing,Li Wenyou,&Zhang Yukui.(2010).Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation.ACTA CHIMICA SINICA,68(23),2421-2428.
MLA Wang Ena,et al."Preparation of Strong Anion-Exchange Chromatographic Support by Atom Transfer Radical Polymerization and Their Application in Protein's Separation".ACTA CHIMICA SINICA 68.23(2010):2421-2428.
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