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Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry
Ding, Li1; Xie, Fuwei1; Xu, Guowang2; Liu, Kejian1; Wang, Sheng1; Xie, Jianping1
关键词Esi/ms Normal-phase Lc Rplc Tobacco Cuticular Components
刊名JOURNAL OF SEPARATION SCIENCE
2010-11-01
DOI10.1002/jssc.201000536
33期:21页:3429-3436
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]2-DIMENSIONAL GAS-CHROMATOGRAPHY ; TRADITIONAL CHINESE MEDICINES ; SUCROSE ESTERS ; ION-EXCHANGE ; MACROCYCLIC DITERPENES ; LABDANIC COMPOUNDS ; GREEK TOBACCO ; CHEMISTRY ; VOLATILE ; CEMBRATRIENETRIOLS
英文摘要Integration of normal-phase LC (NPLC) fractionation with RPLC-ESI/MS was established to detect the polar fractions of cuticular components from Oriental tobacco leaf. NPLC was selected for the fractionation of polar components of cuticular leaf extract, after being concentrated with rotary evaporator, each of the enriched fractions was further analyzed by RPLC-ESI/MS. In total, 83 compounds were finally detected including 45 cembranoids, 15 labdanoids, 20 sucrose esters, and 3 glucose esters (or fructose esters). Three cembranoids and seven labdanoids possibly are new diterpenoids. Glucose esters (or fructose esters) are also reported in Nicotiana tobacco for the first time.
语种英语
WOS记录号WOS:000284230700020
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141673
专题中国科学院大连化学物理研究所
作者单位1.Zhengzhou Tobacco Res Inst CNTC, Key Lab Tobacco Chem, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
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GB/T 7714
Ding, Li,Xie, Fuwei,Xu, Guowang,et al. Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(21):3429-3436.
APA Ding, Li,Xie, Fuwei,Xu, Guowang,Liu, Kejian,Wang, Sheng,&Xie, Jianping.(2010).Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry.JOURNAL OF SEPARATION SCIENCE,33(21),3429-3436.
MLA Ding, Li,et al."Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry".JOURNAL OF SEPARATION SCIENCE 33.21(2010):3429-3436.
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