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题名: Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry
作者: Ding, Li1;  Xie, Fuwei1;  Xu, Guowang2;  Liu, Kejian1;  Wang, Sheng1;  Xie, Jianping1
关键词: ESI/MS ;  Normal-phase LC ;  RPLC ;  Tobacco cuticular components
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2010-11-01
DOI: 10.1002/jssc.201000536
卷: 33, 期:21, 页:3429-3436
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Integration of normal-phase LC (NPLC) fractionation with RPLC-ESI/MS was established to detect the polar fractions of cuticular components from Oriental tobacco leaf. NPLC was selected for the fractionation of polar components of cuticular leaf extract, after being concentrated with rotary evaporator, each of the enriched fractions was further analyzed by RPLC-ESI/MS. In total, 83 compounds were finally detected including 45 cembranoids, 15 labdanoids, 20 sucrose esters, and 3 glucose esters (or fructose esters). Three cembranoids and seven labdanoids possibly are new diterpenoids. Glucose esters (or fructose esters) are also reported in Nicotiana tobacco for the first time.
关键词[WOS]: 2-DIMENSIONAL GAS-CHROMATOGRAPHY ;  TRADITIONAL CHINESE MEDICINES ;  SUCROSE ESTERS ;  ION-EXCHANGE ;  MACROCYCLIC DITERPENES ;  LABDANIC COMPOUNDS ;  GREEK TOBACCO ;  CHEMISTRY ;  VOLATILE ;  CEMBRATRIENETRIOLS
语种: 英语
WOS记录号: WOS:000284230700020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141673
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Zhengzhou Tobacco Res Inst CNTC, Key Lab Tobacco Chem, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Ding, Li,Xie, Fuwei,Xu, Guowang,et al. Separation and detection of polar cuticular components from Oriental tobacco leaf by integration of normal-phase liquid chromatography fractionation with reversed-phase liquid chromatography-mass spectrometry[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(21):3429-3436.
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