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HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods
Gao, Liang1,2; Tao, Dingyin1,2; Shan, Yichu1; Liang, Zhen1; Zhang, Lihua1; Huo, Yushu1; Zhang, Yukui1
关键词Deer Antler Mu Rplc-esi-ms/ms Parallel Protein Extraction Shotgun Proteomic Research Serially Coupled Microcolumn
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2010-12-15
DOI10.1016/j.jchromb.2010.10.022
878期:32页:3370-3374
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CERVUS-ELAPHUS ; LIQUID-CHROMATOGRAPHY ; MATRIX PROTEINS ; BONE ; IDENTIFICATION ; TRANSFORMATION ; BINDING ; GROWTH ; TISSUE
英文摘要Deer antlers mature rapidly in 60 days, and subsequently shed in 5 days with rapid ossification. During this procedure, the function of deer antlers changes significantly. Therefore, the profiling of antler proteome is helpful to discover important growing and shedding regulation proteins, which might be of great significance for studying development and regeneration. In this study, a parallel protein extraction strategy was developed to extract proteins from antlers of red deer with five different lysis solutions, followed by shotgun proteomic analysis by microflow reversed-phase liquid chromatography/electrospray ionization/tandem mass spectrometry (mu RPLC-ESI-MS/MS) with a 30 cm-long serially coupled microcolumn. Our experimental results showed that the identified proteins extracted by five kinds of lysis solution were complementary to each other. In total, 416 unique proteins were identified, with relative molecular masses from 2000 to 600,000, and isoelectric points from 3.84 to 11.57. All these results demonstrate that the combination of parallel protein extraction strategy and mu RPLC-ESI-MS/MS analysis with serially coupled long microcolumns might be of great significance for comprehensive proteomic research of deer antler. (c) 2010 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000285951500007
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141674
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Gao, Liang,Tao, Dingyin,Shan, Yichu,et al. HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(32):3370-3374.
APA Gao, Liang.,Tao, Dingyin.,Shan, Yichu.,Liang, Zhen.,Zhang, Lihua.,...&Zhang, Yukui.(2010).HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(32),3370-3374.
MLA Gao, Liang,et al."HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.32(2010):3370-3374.
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