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题名: HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods
作者: Gao, Liang1, 2;  Tao, Dingyin1, 2;  Shan, Yichu1;  Liang, Zhen1;  Zhang, Lihua1;  Huo, Yushu1;  Zhang, Yukui1
关键词: Deer antler ;  mu RPLC-ESI-MS/MS ;  Parallel protein extraction ;  Shotgun proteomic research ;  Serially coupled microcolumn
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2010-12-15
DOI: 10.1016/j.jchromb.2010.10.022
卷: 878, 期:32, 页:3370-3374
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Deer antlers mature rapidly in 60 days, and subsequently shed in 5 days with rapid ossification. During this procedure, the function of deer antlers changes significantly. Therefore, the profiling of antler proteome is helpful to discover important growing and shedding regulation proteins, which might be of great significance for studying development and regeneration. In this study, a parallel protein extraction strategy was developed to extract proteins from antlers of red deer with five different lysis solutions, followed by shotgun proteomic analysis by microflow reversed-phase liquid chromatography/electrospray ionization/tandem mass spectrometry (mu RPLC-ESI-MS/MS) with a 30 cm-long serially coupled microcolumn. Our experimental results showed that the identified proteins extracted by five kinds of lysis solution were complementary to each other. In total, 416 unique proteins were identified, with relative molecular masses from 2000 to 600,000, and isoelectric points from 3.84 to 11.57. All these results demonstrate that the combination of parallel protein extraction strategy and mu RPLC-ESI-MS/MS analysis with serially coupled long microcolumns might be of great significance for comprehensive proteomic research of deer antler. (c) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: CERVUS-ELAPHUS ;  LIQUID-CHROMATOGRAPHY ;  MATRIX PROTEINS ;  BONE ;  IDENTIFICATION ;  TRANSFORMATION ;  BINDING ;  GROWTH ;  TISSUE
语种: 英语
WOS记录号: WOS:000285951500007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141674
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Gao, Liang,Tao, Dingyin,Shan, Yichu,et al. HPLC-MS/MS shotgun proteomic research of deer antlers with multiparallel protein extraction methods[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(32):3370-3374.
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