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Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum
Gao, Liang1,2; Zhang, Jie1; Zhang, Weibing1,3; Shan, Yichu1; Liang, Zhen1; Zhang, Lihua1; Huo, Yushu1; Zhang, Yukui1
关键词Comprehensive 2-d Chromatography Normal Phase Lc Supercritical Fluid Chromatography Traditional Chinese Medicines
刊名JOURNAL OF SEPARATION SCIENCE
2010-12-01
DOI10.1002/jssc.201000453
33期:23-24页:3817-3821
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SHOTGUN PROTEOME ANALYSIS ; MULTIDIMENSIONAL SEPARATION ; MASS-SPECTROMETRY ; REVERSED-PHASE ; COLUMN ; IDENTIFICATION ; INTERFACE ; SYSTEM ; RPLC
英文摘要In this study, a comprehensive 2-D chromatography was constructed, consisting of normal phase LC (NPLC) with a CN column as the first dimension, and supercritical fluid chromatography (SFC), with a Merck Chromolith Flash C(18) column as the second dimension, which were connected by a 10-port, dual-position valve controlled automatically by a self-designed software. Such platform was applied into the analysis of the fruiting bodies of Ganoderma lucidum, a traditional Chinese medicine, and within 2 h analysis, the obtained peak capacity of the 2-D-NPLC-SFC system was about 350, obviously higher than that of each dimension. These results demonstrate that 2-D-NPLC-SFC is not only of good orthogonality, but also of high throughput for the analysis of complex samples.
语种英语
WOS记录号WOS:000285312700026
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141675
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatograph Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.E China Univ Sci & Technol, Shanghai 200237, Peoples R China
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Gao, Liang,Zhang, Jie,Zhang, Weibing,et al. Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(23-24):3817-3821.
APA Gao, Liang.,Zhang, Jie.,Zhang, Weibing.,Shan, Yichu.,Liang, Zhen.,...&Zhang, Yukui.(2010).Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum.JOURNAL OF SEPARATION SCIENCE,33(23-24),3817-3821.
MLA Gao, Liang,et al."Integration of normal phase liquid chromatography with supercritical fluid chromatography for analysis of fruiting bodies of Ganoderma lucidum".JOURNAL OF SEPARATION SCIENCE 33.23-24(2010):3817-3821.
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