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Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography
Liu, Yanfang1; Feng, Jiatao1; Xiao, Yuansheng1; Guo, Zhimou1; Zhang, Jing1; Xue, Xingya1; Ding, Jin2; Zhang, Xiuli1; Liang, Xinmiao1
关键词Bufadienolides Cyclodextrin Hydrophilic Interaction Chromatography Purification Reversed-phase Liquid Chromatography
刊名JOURNAL OF SEPARATION SCIENCE
2010-05-01
DOI10.1002/jssc.200900848
33期:10页:1487-1494
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]NATURAL-PRODUCT LIBRARIES ; BUFO-BUFO-GARGARIZANS ; DRUG CHAN-SU ; ANTIMICROBIAL ACTIVITY ; CINOBUFAGIN ; SEPARATION ; DISCOVERY ; STEROIDS ; PLANT ; CYTOTOXICITY
英文摘要An isolation method by reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography was successfully used to isolate and purify active bufadienolides from Bufo bufo gargarizans Cantor toad skin. In the first step, crude samples were run on an XTerra Prep C18 column. After screening for activity, two fractions were chosen for further purification. A Click beta-Cyclodextrin (Click-CD) column made in our laboratory was used for the second step. Seven compounds, including four stereoisomers, were obtained at high purity. The orthogonal isolation method described here was a powerful tool for isolating bufadienolides, including stereoisomers, from a natural product. This integrated method may be useful for the discovery of novel active compounds from natural products.
语种英语
WOS记录号WOS:000278572000016
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被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141680
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai, Peoples R China
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Liu, Yanfang,Feng, Jiatao,Xiao, Yuansheng,et al. Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(10):1487-1494.
APA Liu, Yanfang.,Feng, Jiatao.,Xiao, Yuansheng.,Guo, Zhimou.,Zhang, Jing.,...&Liang, Xinmiao.(2010).Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography.JOURNAL OF SEPARATION SCIENCE,33(10),1487-1494.
MLA Liu, Yanfang,et al."Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography".JOURNAL OF SEPARATION SCIENCE 33.10(2010):1487-1494.
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