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Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography
Liu, Yanfang1; Feng, Jiatao1; Xiao, Yuansheng1; Guo, Zhimou1; Zhang, Jing1; Xue, Xingya1; Ding, Jin2; Zhang, Xiuli1; Liang, Xinmiao1
KeywordBufadienolides Cyclodextrin Hydrophilic Interaction Chromatography Purification Reversed-phase Liquid Chromatography
Source PublicationJOURNAL OF SEPARATION SCIENCE
2010-05-01
DOI10.1002/jssc.200900848
Volume33Issue:10Pages:1487-1494
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordNATURAL-PRODUCT LIBRARIES ; BUFO-BUFO-GARGARIZANS ; DRUG CHAN-SU ; ANTIMICROBIAL ACTIVITY ; CINOBUFAGIN ; SEPARATION ; DISCOVERY ; STEROIDS ; PLANT ; CYTOTOXICITY
AbstractAn isolation method by reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography was successfully used to isolate and purify active bufadienolides from Bufo bufo gargarizans Cantor toad skin. In the first step, crude samples were run on an XTerra Prep C18 column. After screening for activity, two fractions were chosen for further purification. A Click beta-Cyclodextrin (Click-CD) column made in our laboratory was used for the second step. Seven compounds, including four stereoisomers, were obtained at high purity. The orthogonal isolation method described here was a powerful tool for isolating bufadienolides, including stereoisomers, from a natural product. This integrated method may be useful for the discovery of novel active compounds from natural products.
Language英语
WOS IDWOS:000278572000016
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141680
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yanfang,Feng, Jiatao,Xiao, Yuansheng,et al. Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(10):1487-1494.
APA Liu, Yanfang.,Feng, Jiatao.,Xiao, Yuansheng.,Guo, Zhimou.,Zhang, Jing.,...&Liang, Xinmiao.(2010).Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography.JOURNAL OF SEPARATION SCIENCE,33(10),1487-1494.
MLA Liu, Yanfang,et al."Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography".JOURNAL OF SEPARATION SCIENCE 33.10(2010):1487-1494.
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