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题名: Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography
作者: Liu, Yanfang1;  Feng, Jiatao1;  Xiao, Yuansheng1;  Guo, Zhimou1;  Zhang, Jing1;  Xue, Xingya1;  Ding, Jin2;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Bufadienolides ;  Cyclodextrin ;  Hydrophilic interaction chromatography ;  Purification ;  Reversed-phase liquid chromatography
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2010-05-01
DOI: 10.1002/jssc.200900848
卷: 33, 期:10, 页:1487-1494
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An isolation method by reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography was successfully used to isolate and purify active bufadienolides from Bufo bufo gargarizans Cantor toad skin. In the first step, crude samples were run on an XTerra Prep C18 column. After screening for activity, two fractions were chosen for further purification. A Click beta-Cyclodextrin (Click-CD) column made in our laboratory was used for the second step. Seven compounds, including four stereoisomers, were obtained at high purity. The orthogonal isolation method described here was a powerful tool for isolating bufadienolides, including stereoisomers, from a natural product. This integrated method may be useful for the discovery of novel active compounds from natural products.
关键词[WOS]: NATURAL-PRODUCT LIBRARIES ;  BUFO-BUFO-GARGARIZANS ;  DRUG CHAN-SU ;  ANTIMICROBIAL ACTIVITY ;  CINOBUFAGIN ;  SEPARATION ;  DISCOVERY ;  STEROIDS ;  PLANT ;  CYTOTOXICITY
语种: 英语
WOS记录号: WOS:000278572000016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141680
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
2.Second Mil Med Univ, Eastern Hepatobiliary Surg Inst, Int Cooperat Lab Signal Transduct, Shanghai, Peoples R China

Recommended Citation:
Liu, Yanfang,Feng, Jiatao,Xiao, Yuansheng,et al. Purification of active bufadienolides from toad skin by preparative reversed-phase liquid chromatography coupled with hydrophilic interaction chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(10):1487-1494.
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