中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Weak anion and cation exchange mixed-bed microcolumn for protein separation
作者: Hou, Chunyan1, 2;  Yuan, Huiming1;  Qiao, Xiaoqiang1, 2;  Liu, Jinxiang1, 2;  Shan, Yichu1;  Zhang, Lihua1;  Liang, Zhen1;  Zhang, Yukui1
关键词: Ion-exchange chromatography ;  Protein separation ;  Tryptic digestion ;  Weak anion and cation exchange mixed-bed
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2010-11-01
DOI: 10.1002/jssc.201000440
卷: 33, 期:21, 页:3299-3303
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: To separate proteins with a wide distribution of pIs under the conditions compatible to online tryptic digestion (with preferable pH = 8.0), weak anion and cation exchange chromatography (WAX/WCX) mixed-bed microcolumn has been developed. With a mixture of five proteins with pIs ranging from 4.2 to 11.4, the effect of WAX/WCX ratio on the separation performance was investigated, and an optimum packing ratio of 1:1 w/w was obtained. Moreover, the undesirable hydrophobic interaction between the proteins and the stationary phase was suppressed with 10% ACN v/v added in the mobile phases. Under the optimized conditions compatible to tryptic digestion, basic and acidic proteins were resolved simultaneously, with RSDs of relative retention time on six columns less than 6%, indicating the good resolution and packing reproducibility. Furthermore, one RPLC fraction of proteins extracted from rat middle brain and the whole protein mixture extracted from rat liver were analyzed, respectively. The results demonstrated better separation performance on WAX/WCX microcolumns than that on both weak anion exchange chromatography and weak cation exchange chromatography at pH similar to 8. We anticipate that WAX/WCX microcolumns are promising for the integration of protein separation and tryptic digestion aiming at high-throughput proteome study.
关键词[WOS]: ION-EXCHANGE ;  LIQUID-CHROMATOGRAPHY ;  MASS-SPECTROMETRY ;  PH ;  SALT
语种: 英语
WOS记录号: WOS:000284230700004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141684
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Hou, Chunyan,Yuan, Huiming,Qiao, Xiaoqiang,et al. Weak anion and cation exchange mixed-bed microcolumn for protein separation[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(21):3299-3303.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Hou, Chunyan]'s Articles
 [Yuan, Huiming]'s Articles
 [Qiao, Xiaoqiang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Hou, Chunyan]‘s Articles
 [Yuan, Huiming]‘s Articles
 [Qiao, Xiaoqiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace