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题名: Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles
作者: Wang, Xinyi2;  Xu, Yao1;  Xu, Xiao1;  Hu, Ke1;  Xiang, Minghui1;  Li, Limei2;  Liu, Feng1;  Li, Na1
关键词: Lysozyme ;  Plasmon Resonance Light-Scattering ;  Gold Nanoparticles ;  Urinary sample
刊名: TALANTA
发表日期: 2010-07-15
DOI: 10.1016/j.talanta.2010.05.034
卷: 82, 期:2, 页:693-697
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: LINKED-IMMUNOSORBENT-ASSAY ;  HUMAN SERUM-ALBUMIN ;  EGG-WHITE ;  PROTEIN ;  CHROMATOGRAPHY ;  BIOSENSOR ;  PROBE ;  ELECTROPHORESIS ;  DYSFUNCTION ;  APTAMER
语种: 英语
WOS记录号: WOS:000280375700040
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141687
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, BNLMS, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China

Recommended Citation:
Wang, Xinyi,Xu, Yao,Xu, Xiao,et al. Direct Determination of Urinary Lysozyme Using Surface Plasmon Resonance Light-Scattering of Gold Nanoparticles[J]. TALANTA,2010,82(2):693-697.
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