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题名: Formation of i-motif structure at neutral and slightly alkaline pH
作者: Zhou, Jun1, 2;  Wei, Chunying3;  Jia, Guoqing1, 2;  Wang, Xiuli1, 2;  Feng, Zhaochi1;  Li, Can1
刊名: MOLECULAR BIOSYSTEMS
发表日期: 2010
DOI: 10.1039/b919600e
卷: 6, 期:3, 页:580-586
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: It is well known that oligonucleotides containing tracts cytosines can form i-motif structures under acidic conditions (pH < 7). However, whether i-motif can be formed under normal physiological cellular conditions (pH 7.0-7.5) is yet no conclusive proof. In the present work, using circular dichroism (CD), UV absorption spectroscopies and native polyacrylamide gel electrophoresis (PAGE), we provided the compelling evidence for the formation of i-motif structures by four cytosine clusters, [C(3)TA(2)](3)C-3 (HT), [C(4)G](3)C(4)TA (RET), C2T3C2T4C2T3C2 (CTC) and GC(2)GC(3)A(4)C(6)G (Rb), at neutral and slightly alkaline pH at 4 degrees C. Furthermore, for HT, we also supplied the evidence for the formation of i-motif structure by fluorescence resonance energy transfer (FRET) and investigated its folding kinetics. The formation time constants obtained by CD and fluorescence experiments are 214 and 493 s, respectively, indicating that HT can slowly form i-motif structure at pH 7.0 and 4 degrees C. This work implies that i-motif structures may possible form in vivo.
关键词[WOS]: C-MYC PROMOTER ;  NUCLEASE HYPERSENSITIVE ELEMENT ;  CYTOSINE-RICH STRAND ;  G-QUADRUPLEX ;  NONCONTACTING MACROMOLECULES ;  TETRAPLEX FORMATION ;  DNA NANOMACHINE ;  TELOMERIC MOTIF ;  ENERGY-TRANSFER ;  HUMAN GENOME
语种: 英语
WOS记录号: WOS:000274654400015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141695
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China

Recommended Citation:
Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Formation of i-motif structure at neutral and slightly alkaline pH[J]. MOLECULAR BIOSYSTEMS,2010,6(3):580-586.
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