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Formation of i-motif structure at neutral and slightly alkaline pH
Zhou, Jun1,2; Wei, Chunying3; Jia, Guoqing1,2; Wang, Xiuli1,2; Feng, Zhaochi1; Li, Can1
刊名MOLECULAR BIOSYSTEMS
2010
DOI10.1039/b919600e
6期:3页:580-586
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]C-MYC PROMOTER ; NUCLEASE HYPERSENSITIVE ELEMENT ; CYTOSINE-RICH STRAND ; G-QUADRUPLEX ; NONCONTACTING MACROMOLECULES ; TETRAPLEX FORMATION ; DNA NANOMACHINE ; TELOMERIC MOTIF ; ENERGY-TRANSFER ; HUMAN GENOME
英文摘要It is well known that oligonucleotides containing tracts cytosines can form i-motif structures under acidic conditions (pH < 7). However, whether i-motif can be formed under normal physiological cellular conditions (pH 7.0-7.5) is yet no conclusive proof. In the present work, using circular dichroism (CD), UV absorption spectroscopies and native polyacrylamide gel electrophoresis (PAGE), we provided the compelling evidence for the formation of i-motif structures by four cytosine clusters, [C(3)TA(2)](3)C-3 (HT), [C(4)G](3)C(4)TA (RET), C2T3C2T4C2T3C2 (CTC) and GC(2)GC(3)A(4)C(6)G (Rb), at neutral and slightly alkaline pH at 4 degrees C. Furthermore, for HT, we also supplied the evidence for the formation of i-motif structure by fluorescence resonance energy transfer (FRET) and investigated its folding kinetics. The formation time constants obtained by CD and fluorescence experiments are 214 and 493 s, respectively, indicating that HT can slowly form i-motif structure at pH 7.0 and 4 degrees C. This work implies that i-motif structures may possible form in vivo.
语种英语
WOS记录号WOS:000274654400015
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被引频次:52[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141695
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
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Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Formation of i-motif structure at neutral and slightly alkaline pH[J]. MOLECULAR BIOSYSTEMS,2010,6(3):580-586.
APA Zhou, Jun,Wei, Chunying,Jia, Guoqing,Wang, Xiuli,Feng, Zhaochi,&Li, Can.(2010).Formation of i-motif structure at neutral and slightly alkaline pH.MOLECULAR BIOSYSTEMS,6(3),580-586.
MLA Zhou, Jun,et al."Formation of i-motif structure at neutral and slightly alkaline pH".MOLECULAR BIOSYSTEMS 6.3(2010):580-586.
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