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Formation of i-motif structure at neutral and slightly alkaline pH
Zhou, Jun1,2; Wei, Chunying3; Jia, Guoqing1,2; Wang, Xiuli1,2; Feng, Zhaochi1; Li, Can1
Source PublicationMOLECULAR BIOSYSTEMS
2010
DOI10.1039/b919600e
Volume6Issue:3Pages:580-586
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiochemistry & Molecular Biology
WOS Research AreaBiochemistry & Molecular Biology
WOS KeywordC-MYC PROMOTER ; NUCLEASE HYPERSENSITIVE ELEMENT ; CYTOSINE-RICH STRAND ; G-QUADRUPLEX ; NONCONTACTING MACROMOLECULES ; TETRAPLEX FORMATION ; DNA NANOMACHINE ; TELOMERIC MOTIF ; ENERGY-TRANSFER ; HUMAN GENOME
AbstractIt is well known that oligonucleotides containing tracts cytosines can form i-motif structures under acidic conditions (pH < 7). However, whether i-motif can be formed under normal physiological cellular conditions (pH 7.0-7.5) is yet no conclusive proof. In the present work, using circular dichroism (CD), UV absorption spectroscopies and native polyacrylamide gel electrophoresis (PAGE), we provided the compelling evidence for the formation of i-motif structures by four cytosine clusters, [C(3)TA(2)](3)C-3 (HT), [C(4)G](3)C(4)TA (RET), C2T3C2T4C2T3C2 (CTC) and GC(2)GC(3)A(4)C(6)G (Rb), at neutral and slightly alkaline pH at 4 degrees C. Furthermore, for HT, we also supplied the evidence for the formation of i-motif structure by fluorescence resonance energy transfer (FRET) and investigated its folding kinetics. The formation time constants obtained by CD and fluorescence experiments are 214 and 493 s, respectively, indicating that HT can slowly form i-motif structure at pH 7.0 and 4 degrees C. This work implies that i-motif structures may possible form in vivo.
Language英语
WOS IDWOS:000274654400015
Citation statistics
Cited Times:56[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141695
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Shanxi Univ, Inst Mol Sci, Minist Educ, Key Lab Chem Biol & Mol Engn, Taiyuan 030006, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Jun,Wei, Chunying,Jia, Guoqing,et al. Formation of i-motif structure at neutral and slightly alkaline pH[J]. MOLECULAR BIOSYSTEMS,2010,6(3):580-586.
APA Zhou, Jun,Wei, Chunying,Jia, Guoqing,Wang, Xiuli,Feng, Zhaochi,&Li, Can.(2010).Formation of i-motif structure at neutral and slightly alkaline pH.MOLECULAR BIOSYSTEMS,6(3),580-586.
MLA Zhou, Jun,et al."Formation of i-motif structure at neutral and slightly alkaline pH".MOLECULAR BIOSYSTEMS 6.3(2010):580-586.
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