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题名: Adsorptive Immobilization of Four Proteases on Different Molecular Sieves
作者: Liu Ping1;  Xing Guo-Wen1;  Li Xuan-Wen1;  Ye Yun-Hua1
关键词: Adsorption ;  Immobilization ;  Protease ;  Zeolite ;  Molecular sieve ;  Peptide synthesis
刊名: ACTA PHYSICO-CHIMICA SINICA
发表日期: 2010-04-01
DOI: 10.3866/PKU.WHXB20100448
卷: 26, 期:4, 页:1113-1118
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Physical
研究领域[WOS]: Chemistry
英文摘要: The immobilization of four different proteases on a series of molecular sieves, including microporous HY, NaY, NH4Y, MCM-22, H beta zeolites, modified Y zeolites HDAY, HNH(4)DAY, and mesoporous MCM-41, by adsorption was systematically investigated The four proteases were alpha-chymotrypsin, papain, subtilisin, and thermoase (or its pure form thermolysin) The results showed that the enzyme loading amount and the activity of immobilized enzyme were significantly affected not only by the structures and textures of molecular sieves and the enzyme properties. but also by the adsorptive conditions such as buffer pH values and enzyme concentration In most cases, the amount of protease loading on molecular sieves was relatively higher at pH 6 0, but declined with further increasing of pH values The nature of the interaction between protease and molecular sieves is discussed As for alpha-chymotrypsin, its loading amount on H beta zeolites was found to be the highest, whereas the activity of a-chymotrypsin immobilized on MCM-22 was the highest, which is probably due to different adsorption states.
关键词[WOS]: ORGANIC-SOLVENTS ;  ENZYMES ;  ZEOLITES ;  SUPPORT ;  MCM-22
语种: 英语
WOS记录号: WOS:000276958400041
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141700
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Liu Ping,Xing Guo-Wen,Li Xuan-Wen,et al. Adsorptive Immobilization of Four Proteases on Different Molecular Sieves[J]. ACTA PHYSICO-CHIMICA SINICA,2010,26(4):1113-1118.
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