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题名: Construction of an Artificial Signal Transduction System on Bilayer Vesicles: Insertion of Transmembrane Peptide of Bacterial Histidine Protein Kinase on Bilayer Vesicles
作者: Quan Chunshan1;  Ma Jinlong1, 2;  Wang Xue3;  Li Wei4;  Fan Shengdi1
关键词: transmembrane transduction ;  Staphyloccocus aureus ;  AgrC ;  transmembrane insertion
刊名: ACTA CHIMICA SINICA
发表日期: 2010-06-28
卷: 68, 期:12, 页:1167-1173
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: One of the six transmembrane domains of AgrC from Staphylococcus aureus ATCC 6538 (agr I type) which is closest with the process of transmembrane signal transduction in quorum sensing was selected as a receptor. This polypeptide was inserted into the artificial cell membrane by ultrasonic procedure, and the pattern of the vesicles was analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). It was found that the single shape of most vesicles (blank and embedded with peptide) was uniform sphere, and the diameter size is from 50 to 200 nm. Considering the impact of suspension pH, concentration and temperature factors to the stability of the embeded system, it is found that the system was the most stable under the following conditions: pH 7.0, temperature lower than 30 degrees C, mole ratio of the polypeptide and N(+)C(5)Ala2C(16) being 1 : 100. Meanwhile, it is also discovered that polypeptide was capable of inserting into bilayer vesicles stably and positively to form embeded system by circular dichroism spectra and differential scanning calorimetry.
关键词[WOS]: STAPHYLOCOCCUS-AUREUS ;  ENZYMATIC-ACTIVITY ;  INTERMOLECULAR COMMUNICATION ;  LIPID SIGNAL ;  RECEPTOR ;  RECOGNITION ;  MEMBRANE ;  AGRC ;  ACTIVATION
语种: 英语
WOS记录号: WOS:000279709400003
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141706
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Nationalities Univ, Coll Life Sci, Dalian 116600, Peoples R China
2.Zhejiang Univ, Dept Chem & Biol Engn, Hangzhou 310027, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116064, Peoples R China

Recommended Citation:
Quan Chunshan,Ma Jinlong,Wang Xue,et al. Construction of an Artificial Signal Transduction System on Bilayer Vesicles: Insertion of Transmembrane Peptide of Bacterial Histidine Protein Kinase on Bilayer Vesicles[J]. ACTA CHIMICA SINICA,2010,68(12):1167-1173.
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