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题名: Micropillar array chips toward new immunodiagnosis
作者: Li, Hong-Yi1, 2;  Dauriac, Virginie2;  Thibert, Valerie2;  Senechal, Helene2;  Peltre, Gabriel2;  Zhang, Xin-Xiang1;  Descroix, Stephanie2
刊名: LAB ON A CHIP
发表日期: 2010
DOI: 10.1039/c005034b
卷: 10, 期:19, 页:2597-2604
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: In this paper, we demonstrate the possibility to use a micropillar array to perform molecular immunodiagnosis. A polydimethylsiloxane (PDMS) microdevice consisting of a rectangular array of micropillars (45 mu m in height, 100 x 100 mu m square cross section) was used to replace microchannels or gels (polyacrylamide or agarose) to perform electrokinetic separation. This microarray was used to mimic highly diluted gel and to maintain electrolyte within the pillar zone by capillary effect. The electrolyte composition (glycerol and agarose content) was investigated in order to improve protein separation by isoelectric focusing (IEF). The influence of glycerol on focusing time and on the different evaporative contributions was further evaluated. In order to perform an immunodiagnostic of milk allergy, different surface treatments were optimized to prevent milk allergen adsorption on PDMS surface. Poly(dimethylacrylamide)-co-allyl glycidyl ether (PDMA-AGE) as well as gelatin led to a satisfactory signal to noise ratio. Finally the possibility to perform protein mixture separation using this micropillar array chip followed by immunoblotting was demonstrated by using the serum from an allergic individual, confirming the great potential of this analytical platform in the field of immunodiagnosis.
关键词[WOS]: TOTAL ANALYSIS SYSTEMS ;  BOVINE BETA-LACTOGLOBULIN ;  ALLERGEN-SPECIFIC IGE ;  CAPILLARY-ELECTROPHORESIS ;  MICROFLUIDIC SYSTEMS ;  CARRIER AMPHOLYTES ;  MILK ALLERGY ;  MICROCHIP ;  SERUM ;  IMMUNOASSAY
语种: 英语
WOS记录号: WOS:000281614900017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141709
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Coll Chem,Minist Educ, Beijing 100871, Peoples R China
2.PECSA, UMR 7195, F-75231 Paris 05, France

Recommended Citation:
Li, Hong-Yi,Dauriac, Virginie,Thibert, Valerie,et al. Micropillar array chips toward new immunodiagnosis[J]. LAB ON A CHIP,2010,10(19):2597-2604.
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