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Micropillar array chips toward new immunodiagnosis
Li, Hong-Yi1,2; Dauriac, Virginie2; Thibert, Valerie2; Senechal, Helene2; Peltre, Gabriel2; Zhang, Xin-Xiang1; Descroix, Stephanie2
刊名LAB ON A CHIP
2010
DOI10.1039/c005034b
10期:19页:2597-2604
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]TOTAL ANALYSIS SYSTEMS ; BOVINE BETA-LACTOGLOBULIN ; ALLERGEN-SPECIFIC IGE ; CAPILLARY-ELECTROPHORESIS ; MICROFLUIDIC SYSTEMS ; CARRIER AMPHOLYTES ; MILK ALLERGY ; MICROCHIP ; SERUM ; IMMUNOASSAY
英文摘要In this paper, we demonstrate the possibility to use a micropillar array to perform molecular immunodiagnosis. A polydimethylsiloxane (PDMS) microdevice consisting of a rectangular array of micropillars (45 mu m in height, 100 x 100 mu m square cross section) was used to replace microchannels or gels (polyacrylamide or agarose) to perform electrokinetic separation. This microarray was used to mimic highly diluted gel and to maintain electrolyte within the pillar zone by capillary effect. The electrolyte composition (glycerol and agarose content) was investigated in order to improve protein separation by isoelectric focusing (IEF). The influence of glycerol on focusing time and on the different evaporative contributions was further evaluated. In order to perform an immunodiagnostic of milk allergy, different surface treatments were optimized to prevent milk allergen adsorption on PDMS surface. Poly(dimethylacrylamide)-co-allyl glycidyl ether (PDMA-AGE) as well as gelatin led to a satisfactory signal to noise ratio. Finally the possibility to perform protein mixture separation using this micropillar array chip followed by immunoblotting was demonstrated by using the serum from an allergic individual, confirming the great potential of this analytical platform in the field of immunodiagnosis.
语种英语
WOS记录号WOS:000281614900017
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141709
专题中国科学院大连化学物理研究所
作者单位1.Peking Univ, Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Coll Chem,Minist Educ, Beijing 100871, Peoples R China
2.PECSA, UMR 7195, F-75231 Paris 05, France
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Li, Hong-Yi,Dauriac, Virginie,Thibert, Valerie,et al. Micropillar array chips toward new immunodiagnosis[J]. LAB ON A CHIP,2010,10(19):2597-2604.
APA Li, Hong-Yi.,Dauriac, Virginie.,Thibert, Valerie.,Senechal, Helene.,Peltre, Gabriel.,...&Descroix, Stephanie.(2010).Micropillar array chips toward new immunodiagnosis.LAB ON A CHIP,10(19),2597-2604.
MLA Li, Hong-Yi,et al."Micropillar array chips toward new immunodiagnosis".LAB ON A CHIP 10.19(2010):2597-2604.
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