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Micropillar array chips toward new immunodiagnosis
Li, Hong-Yi1,2; Dauriac, Virginie2; Thibert, Valerie2; Senechal, Helene2; Peltre, Gabriel2; Zhang, Xin-Xiang1; Descroix, Stephanie2
Source PublicationLAB ON A CHIP
2010
DOI10.1039/c005034b
Volume10Issue:19Pages:2597-2604
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordTOTAL ANALYSIS SYSTEMS ; BOVINE BETA-LACTOGLOBULIN ; ALLERGEN-SPECIFIC IGE ; CAPILLARY-ELECTROPHORESIS ; MICROFLUIDIC SYSTEMS ; CARRIER AMPHOLYTES ; MILK ALLERGY ; MICROCHIP ; SERUM ; IMMUNOASSAY
AbstractIn this paper, we demonstrate the possibility to use a micropillar array to perform molecular immunodiagnosis. A polydimethylsiloxane (PDMS) microdevice consisting of a rectangular array of micropillars (45 mu m in height, 100 x 100 mu m square cross section) was used to replace microchannels or gels (polyacrylamide or agarose) to perform electrokinetic separation. This microarray was used to mimic highly diluted gel and to maintain electrolyte within the pillar zone by capillary effect. The electrolyte composition (glycerol and agarose content) was investigated in order to improve protein separation by isoelectric focusing (IEF). The influence of glycerol on focusing time and on the different evaporative contributions was further evaluated. In order to perform an immunodiagnostic of milk allergy, different surface treatments were optimized to prevent milk allergen adsorption on PDMS surface. Poly(dimethylacrylamide)-co-allyl glycidyl ether (PDMA-AGE) as well as gelatin led to a satisfactory signal to noise ratio. Finally the possibility to perform protein mixture separation using this micropillar array chip followed by immunoblotting was demonstrated by using the serum from an allergic individual, confirming the great potential of this analytical platform in the field of immunodiagnosis.
Language英语
WOS IDWOS:000281614900017
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141709
Collection中国科学院大连化学物理研究所
Affiliation1.Peking Univ, Inst Analyt Chem, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Coll Chem,Minist Educ, Beijing 100871, Peoples R China
2.PECSA, UMR 7195, F-75231 Paris 05, France
Recommended Citation
GB/T 7714
Li, Hong-Yi,Dauriac, Virginie,Thibert, Valerie,et al. Micropillar array chips toward new immunodiagnosis[J]. LAB ON A CHIP,2010,10(19):2597-2604.
APA Li, Hong-Yi.,Dauriac, Virginie.,Thibert, Valerie.,Senechal, Helene.,Peltre, Gabriel.,...&Descroix, Stephanie.(2010).Micropillar array chips toward new immunodiagnosis.LAB ON A CHIP,10(19),2597-2604.
MLA Li, Hong-Yi,et al."Micropillar array chips toward new immunodiagnosis".LAB ON A CHIP 10.19(2010):2597-2604.
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