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题名: Classification of Diabetes Volunteers Based on Plasma Amino Acids Metabolic Profiling
作者: Xiao-Fei, Han1;  Yu-Hong, Huang2;  Long-Xing, Wang1;  Qian-Xu, Yang1;  Hong-Bin, Xiao1;  De-Qin, Zhang2
关键词: Amino acids ;  High performance liquid chromatography ;  Metabolic profiling ;  Diabetes
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2010-05-01
卷: 38, 期:5, 页:697-701
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: To study the relation of plasma amino acids metabolic profiling and diabetes blood sugar level by using metabolomics, an on-line pre-column o-phthalic dicarboxaldehyde ( OPA) derivatization HPLC method for the relative quantitative determination of 21 amino acids in plasma samples was developed. The derivatization procedure was accomplished by an online auto-injector and the signals were detected with a UV detector. Mobile phase A was 10 mmol/L Na(2)HPO(4)-Na(2)B(4)O(7) buffer at pH 7.95, and B was acetonitrile-methanol-water (45:45:10 by volume). The elution program was 5% B at the start and 46% B at the end in 30 min. The 21 amino acids were satisfactorily separated in 27 min. Fifty-one plasma samples from diabetes volunteers were analyzed and were pre-divided into blood sugar ( B. S.) <6.2 group and B. S > 9. 5 group. By SPSS and Matlab software platform, relation analysis was performed by using linear discriminant analysis, stepwise discriminant analysis, and scores plot models. By correlating the levels of blood sugar( B. S.) to 20 amino acids peak areas except glutamine, the results showed that the B. S. <6.2 group was discriminated from the B. S >9.5 group with a total hit ratio of 94.1%, stepwise discriminant analysis ( SDA) model indicated that 7 out of 20 amino acids,i.e., Arg, Cit, Leu, Asp, Asn, Thr and Trp essentially contributed to the above two groups division with the total hit ratio of 90.2%, which maybe have more means as potential marker groups for the early diagnosis of diabetes and in-depth research.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  ORTHOGONAL SIGNAL CORRECTION ;  NUCLEOSIDES ;  DIAGNOSIS ;  URINARY ;  CANCER ;  MARKER
语种: 英语
WOS记录号: WOS:000278208600018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141719
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Tianjin Univ Tradit Chinese Med, Tianjin 300193, Peoples R China

Recommended Citation:
Xiao-Fei, Han,Yu-Hong, Huang,Long-Xing, Wang,et al. Classification of Diabetes Volunteers Based on Plasma Amino Acids Metabolic Profiling[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(5):697-701.
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