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题名: Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis
作者: Liang, Yuan1;  Zhou, Shuang1;  Hu, Liming1;  Li, Lin1;  Zhao, Meiping1;  Liu, Huwei1
关键词: Immunoaffinity monolith ;  Class-specific ;  Pyrethroid ;  On-line clean-up
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2010-01-15
DOI: 10.1016/j.jchromb.2009.06.007
卷: 878, 期:2, 页:278-282
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A class-specific monolithic immunoaffinity column was developed for on-line clean-up of pyrethroid insecticides in complex samples Deltamethrin was oxidized with ozone to generate the hapten of (RS)-acyano-alpha-phenoxybenzyl (RS)-cis,traps-2,2-dimethyl-3-formyl-cyclopropane carboxylate Class-specific antibodies against pyrethroids were produced using the conjugate of above hapten with bovine serum albumin as the immunogen. Poly(ethylene dimethacrylate-glycidyl methacrylate) monolith was synthesized in a 50 mm, 4.6 mm id stainless steel cartridge with two auxiliary pipette tips. The polymerization method was proved to be economic and reproducible. Antibodies against pyrethrotds were covalently immobilized onto the monolithic support via Schiff base reaction With a column-switching valve system, the immunoaffinity monolith (IAM) could be readily adapted to the reversed-phase high-performance liquid chromatography (HPLC) system. Under the optimum loading, washing and eluting conditions, the IAM specifically retained deltamethrin, flumethrin. flucythrinate and cis/traps permethrin, which were further baseline separated by C18 column using acetonitrile-water (83:17, V/V) as the mobile phase at a flow rate of 1.0 mL(min. The established system provides a highly efficient approach for high-throughput on-line clean-up of pyrethroid in various samples. (C) 2009 Elsevier B.V. All rights reserved.
关键词[WOS]: SOLID-PHASE MICROEXTRACTION ;  GAS-CHROMATOGRAPHY ;  ENZYME-IMMUNOASSAY ;  MASS-SPECTROMETRY ;  ELECTRON-CAPTURE ;  INSECTICIDES ;  PESTICIDES ;  EXTRACTION ;  OPTIMIZATION ;  PERMETHRIN
语种: 英语
WOS记录号: WOS:000274220900022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141742
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, BNLMS,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Liang, Yuan,Zhou, Shuang,Hu, Liming,et al. Class-specific immunoaffinity monolith for efficient on-line clean-up of pyrethroids followed by high-performance liquid chromatography analysis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(2):278-282.
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