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题名: The Effect of Tetramethylpyrazine on Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells
作者: Li, Wen-Ming1;  Liu, Hong-Tao1, 2;  Li, Xiu-Ying1;  Wu, Jian-Yong1;  Xu, Gang1;  Teng, Yong-Zhen1;  Ding, Song-Tao1;  Yu, Chao1
刊名: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
发表日期: 2010
DOI: 10.1111/j.1742-7843.2009.00470.x
卷: 106, 期:1, 页:45-52
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Pharmacology & Pharmacy ;  Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ;  Toxicology
英文摘要: Tetramethylpyrazine has been widely used in traditional Chinese medicine to treat cardiovascular diseases such as atherosclerosis and hypertension. The underlying mechanism of cardioprotective effects, however, remains to be elucidated. Here, using human umbilical vein endothelial cells (HUVECs), we have assessed the protective effect of tetramethylpyrazine on H(2)O(2)-induced oxidative damage. After pre-incubation with tetramethylpyrazine (50, 100 and 150 mu g/ml) for 24 hr., viability loss in H(2)O(2)-induced HUVECs (76.5% of the control level, p < 0.05, at 400 mu M of H(2)O(2) for 12 hr.) was restored in a concentration-dependent manner, and the maximal recovery (88.7% of the control level, p < 0.05) was achieved with tetramethylpyrazine at 150 mu g/ml. The production of reactive oxygen species was suppressed by measuring fluorescent intensity of 2',7'-dichorofluorescein (83.1% of the H(2)O(2)-treated group, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Tetramethylpyrazine also increased activities of superoxide dismutase and glutathione peroxidase (144.1% and 118.3% of the H(2)O(2)-treated group, respectively, p < 0.05 at 150 mu g/ml of tetramethylpyrazine). In addition, tetramethylpyrazine reduced levels of malonaldehyde, intracellular nitric oxide and nitric oxide synthase (83.8%. 91.2% and 78.7% of the H(2)O(2)-treated group, respectively, p < 0.05, at 150 mu g/ml of tetramethylpyrazine). Furthermore, pre-incubation of tetramethylpyrazine with HUVECs for 24 hr. resulted in reduction of apoptosis and removal of cell cycle arrest in the S phase (56.6% and 59.7% of the H(2)O(2)-treated group, respectively, p < 0.01, at 150 mu g/ml of tetramethylpyrazine). Altogether, these results suggest that tetramethylpyrazine has it protective effect oil H(2)O(2)-induced oxidative damage in HUVECs due to its antioxidant and antiapoptotic properties.
关键词[WOS]: NITRIC-OXIDE SYNTHASE ;  PC12 CELLS ;  IN-VITRO ;  H2O2-INDUCED APOPTOSIS ;  ANTIOXIDANT ACTIVITIES ;  EXPRESSION ;  INJURY ;  ACID ;  PROLIFERATION ;  GENERATION
语种: 英语
WOS记录号: WOS:000273297200006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141761
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Li, Wen-Ming,Liu, Hong-Tao,Li, Xiu-Ying,et al. The Effect of Tetramethylpyrazine on Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells[J]. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY,2010,106(1):45-52.
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