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Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling
Wang, Li1; Fan, Jiangli1; Qiao, Xiaoqiang2; Peng, Xiaojun1; Dai, Bin1; Wang, Bingshuai1; Sun, Shiguo1; Zhang, Lihua2; Zhang, Yukui2
关键词Cy5 Cyanine Dye Photophysical Property Photostability Fluorescent Labeling High Performance Liquid Chromatography
刊名JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
2010-02-25
DOI10.1016/j.jphotochem.2010.01.002
210期:2-3页:168-172
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Physical
研究领域[WOS]Chemistry
关键词[WOS]QUANTUM YIELDS ; FLUOROCHROME
英文摘要Several novel water-soluble asymmetric pentamethine cyanine dyes were synthesized. The maximum absorption and emission wavelengths of the dyes in different solvents were in the range from 647 to 665 nm and exhibited negative solvatochromism with increasing solvent polarity. The fluorescence quantum yields of the dyes were about 0.1 in water, and were obviously higher than those of hydrophobic cyanine dyes. Dyes with N-benzyl groups and N-sulfo-groups displayed greater photostability than dyes with N-carboxypentyl groups in water. The limit of detection of dye 5a for BSA was 1.2 x 10(-8) mol L(-1) by high performance liquid chromatography with fluorescent director about 100-fold lower than that by UV detection (1.0 x 10(-6) mol L(-1)). Therefore, Dye 5a could be used to improve photostability and detection sensitivity in protein analysis. (C) 2010 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000276328100011
引用统计
被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141763
专题中国科学院大连化学物理研究所
作者单位1.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Wang, Li,Fan, Jiangli,Qiao, Xiaoqiang,et al. Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling[J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,2010,210(2-3):168-172.
APA Wang, Li.,Fan, Jiangli.,Qiao, Xiaoqiang.,Peng, Xiaojun.,Dai, Bin.,...&Zhang, Yukui.(2010).Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling.JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY,210(2-3),168-172.
MLA Wang, Li,et al."Novel asymmetric Cy5 dyes: Synthesis, photostabilities and high sensitivity in protein fluorescence labeling".JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY 210.2-3(2010):168-172.
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