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Molecular cloning, prokaryotic expression, and purification of an alternatively spliced oligochitosan-induced Ser/Thr protein kinase in tobacco
Yang, Jinli1,2; Yin, Heng1; Lu, Hang1,2; Zhao, Xiaoming1; Du, Yuguang1
KeywordOligochitosan Ser/thr Protein Kinase Alternative Splicing Tobacco Resistance
Source PublicationISRAEL JOURNAL OF PLANT SCIENCES
2010
DOI10.1560/IJPS.58.1.67
Volume58Issue:1Pages:67-75
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectPlant Sciences
WOS Research AreaPlant Sciences
WOS KeywordMOSAIC-VIRUS RESISTANCE ; DEFENSE RESPONSES ; PLANT-DEFENSE ; DISEASE RESISTANCE ; OXIDATIVE BURST ; GENE ; CHITOSAN ; ARABIDOPSIS ; ACTIVATION ; ELICITOR
AbstractOligochitosan elicitor has been reported to induce a variety of defense responses in plants. In our previous work, OIPK (oligochitosan induced protein kinase), which was a Ser/Thr protein kinase, was identified to be induced by oligochitosan through mRNA differential display. When OIPK was repressed in transgenic tobacco, decreased resistance to tobacco mosaic virus (TMV) was observed, which suggested that OIPK might play a role in resistance to TMV in tobacco. To investigate how OIPK functions in oligochitosan-induced tobacco resistance, here we cloned two spliced variants of OIPK gene: OIPKL and OIPKS. The OIPK and OIPKS sequences were almost identical with the OIPKL sequence, except that they lack some segments compared to OIPKL. The full-length OIPKL cDNA was inserted into pET-23b vector, prokaryotically expressed, and purified from inclusion bodies in E. coli strain BL21 (DE3). An anti-His antibody was able to recognize the His-tag in the OIPKL fusion protein, as revealed by immunoblotting. This prokaryotically expressed product could serve as a useful tool for further research on OIPK function in the signal transduction in tobacco resistance.
Language英语
WOS IDWOS:000285721100009
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141766
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Liaoning Prov Key Lab Carbohydrates, Dept Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Yang, Jinli,Yin, Heng,Lu, Hang,et al. Molecular cloning, prokaryotic expression, and purification of an alternatively spliced oligochitosan-induced Ser/Thr protein kinase in tobacco[J]. ISRAEL JOURNAL OF PLANT SCIENCES,2010,58(1):67-75.
APA Yang, Jinli,Yin, Heng,Lu, Hang,Zhao, Xiaoming,&Du, Yuguang.(2010).Molecular cloning, prokaryotic expression, and purification of an alternatively spliced oligochitosan-induced Ser/Thr protein kinase in tobacco.ISRAEL JOURNAL OF PLANT SCIENCES,58(1),67-75.
MLA Yang, Jinli,et al."Molecular cloning, prokaryotic expression, and purification of an alternatively spliced oligochitosan-induced Ser/Thr protein kinase in tobacco".ISRAEL JOURNAL OF PLANT SCIENCES 58.1(2010):67-75.
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