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题名: Properties of i-Motif Under Molecular Crowding Conditions
作者: Zhou Jun1, 2;  Jia Guo-Qing1, 2;  Feng Zhao-Chi1;  Li Can1
关键词: i-Motif ;  Molecular crowding ;  Polyethylene glycol(PEG)
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2010-02-10
卷: 31, 期:2, 页:309-311
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: i-Motif is formed from two parallel duplexes by intercalating with each other in an antiparallel orientation and each duplex is held together via hemiprotonated cytosine(+)-cytosine base pairs. However, a cell is crowded with various biomolecules and little is known about the properties of i-Motif under molecular crowding conditions until now. In the present study, we used human telomeric DNA sequence, [C(3)TA(2)](3)C(3), as a model system to investigate such problem by circular dichroism( CD) and UV absorbance spectroscopy. Based on the CD spectra, we found that there were no changes about the structure of i-Motif in the presence of polyethylene glycol( PEG). CD melting results showed that the thermal stability of i-Motif was increased under molecular crowding conditions compare to that in dilute buffer, which was further demonstrated by UV-melting results. This work suggests that molecular crowding could not affect the structure of i-Motif, which may depend on pH, while could enhance the thermal stability of i-Motif.
关键词[WOS]: G-QUADRUPLEX ;  DNA ;  SEQUENCES
语种: 英语
WOS记录号: WOS:000275476600019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141770
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,et al. Properties of i-Motif Under Molecular Crowding Conditions[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2010,31(2):309-311.
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