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Properties of i-Motif Under Molecular Crowding Conditions
Zhou Jun1,2; Jia Guo-Qing1,2; Feng Zhao-Chi1; Li Can1
关键词I-motif Molecular Crowding Polyethylene Glycol(Peg)
刊名CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
2010-02-10
31期:2页:309-311
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]G-QUADRUPLEX ; DNA ; SEQUENCES
英文摘要i-Motif is formed from two parallel duplexes by intercalating with each other in an antiparallel orientation and each duplex is held together via hemiprotonated cytosine(+)-cytosine base pairs. However, a cell is crowded with various biomolecules and little is known about the properties of i-Motif under molecular crowding conditions until now. In the present study, we used human telomeric DNA sequence, [C(3)TA(2)](3)C(3), as a model system to investigate such problem by circular dichroism( CD) and UV absorbance spectroscopy. Based on the CD spectra, we found that there were no changes about the structure of i-Motif in the presence of polyethylene glycol( PEG). CD melting results showed that the thermal stability of i-Motif was increased under molecular crowding conditions compare to that in dilute buffer, which was further demonstrated by UV-melting results. This work suggests that molecular crowding could not affect the structure of i-Motif, which may depend on pH, while could enhance the thermal stability of i-Motif.
语种英语
WOS记录号WOS:000275476600019
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141770
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,et al. Properties of i-Motif Under Molecular Crowding Conditions[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2010,31(2):309-311.
APA Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,&Li Can.(2010).Properties of i-Motif Under Molecular Crowding Conditions.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,31(2),309-311.
MLA Zhou Jun,et al."Properties of i-Motif Under Molecular Crowding Conditions".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 31.2(2010):309-311.
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