Properties of i-Motif Under Molecular Crowding Conditions
Zhou Jun1,2; Jia Guo-Qing1,2; Feng Zhao-Chi1; Li Can1
KeywordI-motif Molecular Crowding Polyethylene Glycol(Peg)
Indexed BySCI
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Multidisciplinary
WOS Research AreaChemistry
Abstracti-Motif is formed from two parallel duplexes by intercalating with each other in an antiparallel orientation and each duplex is held together via hemiprotonated cytosine(+)-cytosine base pairs. However, a cell is crowded with various biomolecules and little is known about the properties of i-Motif under molecular crowding conditions until now. In the present study, we used human telomeric DNA sequence, [C(3)TA(2)](3)C(3), as a model system to investigate such problem by circular dichroism( CD) and UV absorbance spectroscopy. Based on the CD spectra, we found that there were no changes about the structure of i-Motif in the presence of polyethylene glycol( PEG). CD melting results showed that the thermal stability of i-Motif was increased under molecular crowding conditions compare to that in dilute buffer, which was further demonstrated by UV-melting results. This work suggests that molecular crowding could not affect the structure of i-Motif, which may depend on pH, while could enhance the thermal stability of i-Motif.
WOS IDWOS:000275476600019
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,et al. Properties of i-Motif Under Molecular Crowding Conditions[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2010,31(2):309-311.
APA Zhou Jun,Jia Guo-Qing,Feng Zhao-Chi,&Li Can.(2010).Properties of i-Motif Under Molecular Crowding Conditions.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,31(2),309-311.
MLA Zhou Jun,et al."Properties of i-Motif Under Molecular Crowding Conditions".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 31.2(2010):309-311.
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