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题名: A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis
作者: Pan, Xiaoyan1, 2;  Jiang, Lei1;  Liu, Kaiying1;  Lin, Bingcheng1;  Qin, Jianhua1
关键词: Microfluidic ;  Parallel analysis ;  Multichamber polymerase chain reaction ;  Pathogen detection ;  Genotyping
刊名: ANALYTICA CHIMICA ACTA
发表日期: 2010-07-26
DOI: 10.1016/j.aca.2010.06.005
卷: 674, 期:1, 页:110-115
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: This work describes a microfluidic device integrated with multichamber polymerase chain reaction (PCR) and multichannel separation for parallel genetic analysis. The microdevice consists of three functional units: temperature control, multiple PCR (four chambers PCR), and multiple channel separation (four separation channels, each channel connected to a PCR chamber). Platinum (Pt)/titanium (Ti) microheater was used to ensure homogeneous temperature field, and Pt-chip sensor was used for temperature monitoring. The interface between chip-PCR and chip separation was simplified by connecting the PCR chamber with separation channel directly. After chip-PCR, PCR products were introduced into parallel separation channels for subsequent separation/detection by applying an electric field automatically. This microdevice was successfully applied for detection of pathogens including hepatitis B virus (HBV) and Mycobacterium tuberculosis (MTB), and genotyping of human leucocyte antigen (HLA)-B27 as well, demonstrating the feasibility of the integrated microdevice for parallel genetic analysis. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: CAPILLARY ELECTROPHORESIS MICROSYSTEM ;  ANALYSIS SYSTEM ;  PCR AMPLIFICATION ;  DNA AMPLIFICATION ;  CHIP ;  MICRODEVICE ;  EXTRACTION ;  MICROCHIP ;  SAMPLES
语种: 英语
WOS记录号: WOS:000280570400016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141790
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Pan, Xiaoyan,Jiang, Lei,Liu, Kaiying,et al. A microfluidic device integrated with multichamber polymerase chain reaction and multichannel separation for genetic analysis[J]. ANALYTICA CHIMICA ACTA,2010,674(1):110-115.
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