中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion
作者: Sun, Liangliang1, 2;  Ma, Junfeng1, 2;  Qiao, Xiaoqiang1, 2;  Liang, Yu1, 2;  Zhu, Guijie1;  Shan, Yichu1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2010-03-15
DOI: 10.1021/ac902835p
卷: 82, 期:6, 页:2574-2579
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An integrated sample treatment device, composed of a membrane interface and a monolithic hybrid silica based immobilized enzymatic reactor (IMER), was developed for the simultaneous sample buffer exchange, protein enrichment, and online digestion, by which for the sample buffer, the acetonitrile content was reduced to similar to 1/10 of the initial one, and the pH value was adjusted from similar to 3.0 to similar to 8.0, compatible for online trypsin digestion. Furthermore, the signal intensity of myoglobin digests was improved by over 10 times. Such an integrated device was successfully applied to the online treatment of three protein eluates obtained by reverse-phase liquid chromatography (RPLC) separation, followed by further protein digest analysis with microreverse-phase liquid chromatography-electrospray ionization-tandem mass spectrometry (,mu RPLC-ESI-MS/MS). The experimental results showed that the performance of such an integrated sample treatment device was comparable to that of the traditional offline sample treatment method, including lyophilization and in-solution digestion. However, the consumed time was reduced to 1/192. All these results,demonstrate that such an integrated sample treatment device could be further online coupled with protein separation, peptide separation, and identification, to achieve high-throughput proteome analysis.
关键词[WOS]: MASS-SPECTROMETRY ;  CAPILLARY-ELECTROPHORESIS ;  BOTTOM-UP ;  TOP-DOWN ;  IDENTIFICATION ;  SEPARATION ;  MS ;  MICRODIALYSIS ;  MICROREACTOR ;  PROTEOMICS
语种: 英语
WOS记录号: WOS:000275379300056
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141801
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Liangliang,Ma, Junfeng,Qiao, Xiaoqiang,et al. Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion[J]. ANALYTICAL CHEMISTRY,2010,82(6):2574-2579.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Sun, Liangliang]'s Articles
 [Ma, Junfeng]'s Articles
 [Qiao, Xiaoqiang]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Sun, Liangliang]‘s Articles
 [Ma, Junfeng]‘s Articles
 [Qiao, Xiaoqiang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace