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Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion
Sun, Liangliang1,2; Ma, Junfeng1,2; Qiao, Xiaoqiang1,2; Liang, Yu1,2; Zhu, Guijie1; Shan, Yichu1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
Source PublicationANALYTICAL CHEMISTRY
2010-03-15
DOI10.1021/ac902835p
Volume82Issue:6Pages:2574-2579
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; CAPILLARY-ELECTROPHORESIS ; BOTTOM-UP ; TOP-DOWN ; IDENTIFICATION ; SEPARATION ; MS ; MICRODIALYSIS ; MICROREACTOR ; PROTEOMICS
AbstractAn integrated sample treatment device, composed of a membrane interface and a monolithic hybrid silica based immobilized enzymatic reactor (IMER), was developed for the simultaneous sample buffer exchange, protein enrichment, and online digestion, by which for the sample buffer, the acetonitrile content was reduced to similar to 1/10 of the initial one, and the pH value was adjusted from similar to 3.0 to similar to 8.0, compatible for online trypsin digestion. Furthermore, the signal intensity of myoglobin digests was improved by over 10 times. Such an integrated device was successfully applied to the online treatment of three protein eluates obtained by reverse-phase liquid chromatography (RPLC) separation, followed by further protein digest analysis with microreverse-phase liquid chromatography-electrospray ionization-tandem mass spectrometry (,mu RPLC-ESI-MS/MS). The experimental results showed that the performance of such an integrated sample treatment device was comparable to that of the traditional offline sample treatment method, including lyophilization and in-solution digestion. However, the consumed time was reduced to 1/192. All these results,demonstrate that such an integrated sample treatment device could be further online coupled with protein separation, peptide separation, and identification, to achieve high-throughput proteome analysis.
Language英语
WOS IDWOS:000275379300056
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141801
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Sun, Liangliang,Ma, Junfeng,Qiao, Xiaoqiang,et al. Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion[J]. ANALYTICAL CHEMISTRY,2010,82(6):2574-2579.
APA Sun, Liangliang.,Ma, Junfeng.,Qiao, Xiaoqiang.,Liang, Yu.,Zhu, Guijie.,...&Zhang, Yukui.(2010).Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion.ANALYTICAL CHEMISTRY,82(6),2574-2579.
MLA Sun, Liangliang,et al."Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion".ANALYTICAL CHEMISTRY 82.6(2010):2574-2579.
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