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Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion
Sun, Liangliang1,2; Ma, Junfeng1,2; Qiao, Xiaoqiang1,2; Liang, Yu1,2; Zhu, Guijie1; Shan, Yichu1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
刊名ANALYTICAL CHEMISTRY
2010-03-15
DOI10.1021/ac902835p
82期:6页:2574-2579
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; CAPILLARY-ELECTROPHORESIS ; BOTTOM-UP ; TOP-DOWN ; IDENTIFICATION ; SEPARATION ; MS ; MICRODIALYSIS ; MICROREACTOR ; PROTEOMICS
英文摘要An integrated sample treatment device, composed of a membrane interface and a monolithic hybrid silica based immobilized enzymatic reactor (IMER), was developed for the simultaneous sample buffer exchange, protein enrichment, and online digestion, by which for the sample buffer, the acetonitrile content was reduced to similar to 1/10 of the initial one, and the pH value was adjusted from similar to 3.0 to similar to 8.0, compatible for online trypsin digestion. Furthermore, the signal intensity of myoglobin digests was improved by over 10 times. Such an integrated device was successfully applied to the online treatment of three protein eluates obtained by reverse-phase liquid chromatography (RPLC) separation, followed by further protein digest analysis with microreverse-phase liquid chromatography-electrospray ionization-tandem mass spectrometry (,mu RPLC-ESI-MS/MS). The experimental results showed that the performance of such an integrated sample treatment device was comparable to that of the traditional offline sample treatment method, including lyophilization and in-solution digestion. However, the consumed time was reduced to 1/192. All these results,demonstrate that such an integrated sample treatment device could be further online coupled with protein separation, peptide separation, and identification, to achieve high-throughput proteome analysis.
语种英语
WOS记录号WOS:000275379300056
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被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141801
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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GB/T 7714
Sun, Liangliang,Ma, Junfeng,Qiao, Xiaoqiang,et al. Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion[J]. ANALYTICAL CHEMISTRY,2010,82(6):2574-2579.
APA Sun, Liangliang.,Ma, Junfeng.,Qiao, Xiaoqiang.,Liang, Yu.,Zhu, Guijie.,...&Zhang, Yukui.(2010).Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion.ANALYTICAL CHEMISTRY,82(6),2574-2579.
MLA Sun, Liangliang,et al."Integrated Device for Online Sample Buffer Exchange, Protein Enrichment, and Digestion".ANALYTICAL CHEMISTRY 82.6(2010):2574-2579.
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