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Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis
Ma, Junfeng1,2,3; Hou, Chunyan1,2,3; Sun, Liangliang1,2,3; Tao, Dingyin1,2,3; Zhang, Yanyan2,3; Shan, Yichu1,2; Liang, Zhen1,2; Zhang, Lihua1,2; Yang, Ling2; Zhang, Yukui1,2
刊名ANALYTICAL CHEMISTRY
2010-12-01
DOI10.1021/ac1023099
82期:23页:9622-9625
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PROTEINS ; MS/MS ; IDENTIFICATION ; SURFACTANT ; MS
英文摘要In this study, a facile system for membrane proteome profiling was established, in which membrane proteins were solubilized by formic acid, online digested by a pepsin-based immobilized enzyme reactor (pepsin IMER), and analyzed by strong cation exchange and microflow reversed phase liquid chromatography with electrospray ionization tandem mass spectrometry (SCX-mu RPLC-ESI-MS/MS) Under optimized conditions, such a system showed excellent compatibility between all crucial steps and was successfully applied for analyzing integral membrane proteins extracted from rat liver microsomes Out of the 235 unique proteins positively identified, 39% (91/235) were annotated as membrane proteins with one or more transmembrane domains (TMDs) It is anticipated that the efficient sample treatment and the relevant online analytical system might provide a promising tool for automated and comprehensive profiling of membrane proteomes
语种英语
WOS记录号WOS:000284668600007
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141823
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Ma, Junfeng,Hou, Chunyan,Sun, Liangliang,et al. Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2010,82(23):9622-9625.
APA Ma, Junfeng.,Hou, Chunyan.,Sun, Liangliang.,Tao, Dingyin.,Zhang, Yanyan.,...&Zhang, Yukui.(2010).Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis.ANALYTICAL CHEMISTRY,82(23),9622-9625.
MLA Ma, Junfeng,et al."Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis".ANALYTICAL CHEMISTRY 82.23(2010):9622-9625.
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