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题名: Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis
作者: Ma, Junfeng1, 2, 3;  Hou, Chunyan1, 2, 3;  Sun, Liangliang1, 2, 3;  Tao, Dingyin1, 2, 3;  Zhang, Yanyan2, 3;  Shan, Yichu1, 2;  Liang, Zhen1, 2;  Zhang, Lihua1, 2;  Yang, Ling2;  Zhang, Yukui1, 2
刊名: ANALYTICAL CHEMISTRY
发表日期: 2010-12-01
DOI: 10.1021/ac1023099
卷: 82, 期:23, 页:9622-9625
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: In this study, a facile system for membrane proteome profiling was established, in which membrane proteins were solubilized by formic acid, online digested by a pepsin-based immobilized enzyme reactor (pepsin IMER), and analyzed by strong cation exchange and microflow reversed phase liquid chromatography with electrospray ionization tandem mass spectrometry (SCX-mu RPLC-ESI-MS/MS) Under optimized conditions, such a system showed excellent compatibility between all crucial steps and was successfully applied for analyzing integral membrane proteins extracted from rat liver microsomes Out of the 235 unique proteins positively identified, 39% (91/235) were annotated as membrane proteins with one or more transmembrane domains (TMDs) It is anticipated that the efficient sample treatment and the relevant online analytical system might provide a promising tool for automated and comprehensive profiling of membrane proteomes
关键词[WOS]: PROTEINS ;  MS/MS ;  IDENTIFICATION ;  SURFACTANT ;  MS
语种: 英语
WOS记录号: WOS:000284668600007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141823
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Ma, Junfeng,Hou, Chunyan,Sun, Liangliang,et al. Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2010,82(23):9622-9625.
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