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Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis
Ma, Junfeng1,2,3; Hou, Chunyan1,2,3; Sun, Liangliang1,2,3; Tao, Dingyin1,2,3; Zhang, Yanyan2,3; Shan, Yichu1,2; Liang, Zhen1,2; Zhang, Lihua1,2; Yang, Ling2; Zhang, Yukui1,2
Source PublicationANALYTICAL CHEMISTRY
2010-12-01
DOI10.1021/ac1023099
Volume82Issue:23Pages:9622-9625
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordPROTEINS ; MS/MS ; IDENTIFICATION ; SURFACTANT ; MS
AbstractIn this study, a facile system for membrane proteome profiling was established, in which membrane proteins were solubilized by formic acid, online digested by a pepsin-based immobilized enzyme reactor (pepsin IMER), and analyzed by strong cation exchange and microflow reversed phase liquid chromatography with electrospray ionization tandem mass spectrometry (SCX-mu RPLC-ESI-MS/MS) Under optimized conditions, such a system showed excellent compatibility between all crucial steps and was successfully applied for analyzing integral membrane proteins extracted from rat liver microsomes Out of the 235 unique proteins positively identified, 39% (91/235) were annotated as membrane proteins with one or more transmembrane domains (TMDs) It is anticipated that the efficient sample treatment and the relevant online analytical system might provide a promising tool for automated and comprehensive profiling of membrane proteomes
Language英语
WOS IDWOS:000284668600007
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141823
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Ma, Junfeng,Hou, Chunyan,Sun, Liangliang,et al. Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis[J]. ANALYTICAL CHEMISTRY,2010,82(23):9622-9625.
APA Ma, Junfeng.,Hou, Chunyan.,Sun, Liangliang.,Tao, Dingyin.,Zhang, Yanyan.,...&Zhang, Yukui.(2010).Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis.ANALYTICAL CHEMISTRY,82(23),9622-9625.
MLA Ma, Junfeng,et al."Coupling Formic Acid Assisted Solubilization and Online Immobilized Pepsin Digestion with Strong Cation Exchange and Microflow Reversed-Phase Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for Integral Membrane Proteome Analysis".ANALYTICAL CHEMISTRY 82.23(2010):9622-9625.
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