中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: MALDI-TOF-MS for Lipidomics Analysis - Potential Biomarker for Cadmium Effect on Crucian Carp(Carassius auratus)
作者: Li Guo-Chen1;  Wang Yan-Hong1;  Wu Ren-An2;  Wang Shi-Cheng1
关键词: Cadmium ;  Phospholipids ;  Biomarker ;  Lipidomics ;  MALDI-TOF-MS
刊名: CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
发表日期: 2010-02-10
卷: 31, 期:2, 页:269-273
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The biochemical effects of cadmium(Cd) on Crucian Carp was studied by MALDI-TOF-MS-based lipidomics. Crucian Carps were exposed to water with 500 mu g/L Cd 2, for 0, 10, 20 d, respectively. Muscle samples from Cd-treated fishes were analyzed either by Atomic absorption spectrophotometer(AAS) for Cd contents, or analyzed by HPLC and MALDI-TOF-MS for phospholipids. All MALDI-TOF-MS spectra data were analyzed by pattern recognition using partial least square-discriminant analysis(PLS-DA). During the whole exposure period, Cd contents in muscle kept increasing, while phosphatidylcholine(PC) contents after 10 d exposure were significantly lower than that of normal level(no exposure) (p < 0.05), and the PC contents after 20 d exposure were nor significantly different from the normal level. The three groups were discriminated successfullyby PLS-DA, which suggested that lipidomics studies could better reflect the biochemical effects of Cd. PC could be the biomarker indicating the toxicity of Cd to Crucian Carp. Offline combination of HPLC and MALDI-TOF-MS could not only give the quantify PC, but also isolate and purify PC substraction, hereby it was applicable for lipidomics analysis.
关键词[WOS]: ASSISTED LASER-DESORPTION ;  FLIGHT MASS-SPECTROMETRY ;  MATRIX ;  CHROMATOGRAPHY ;  PHOSPHOLIPIDS ;  DESORPTION/IONIZATION ;  PEPTIDES ;  PROTEINS ;  LIPIDS
语种: 英语
WOS记录号: WOS:000275476600012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141857
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China
2.Chinese Acad Sci, Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Li Guo-Chen,Wang Yan-Hong,Wu Ren-An,et al. MALDI-TOF-MS for Lipidomics Analysis - Potential Biomarker for Cadmium Effect on Crucian Carp(Carassius auratus)[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2010,31(2):269-273.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Li Guo-Chen]'s Articles
 [Wang Yan-Hong]'s Articles
 [Wu Ren-An]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Li Guo-Chen]‘s Articles
 [Wang Yan-Hong]‘s Articles
 [Wu Ren-An]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace