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题名: Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde
作者: Liu, Xing-yu1, 2;  Yang, Zhi-hua3;  Pan, Xiu-jie3;  Zhu, Mao-xiang3;  Xie, Jian-ping1
关键词: Crotonaldehyde ;  Gene expression profile ;  Microarray
刊名: TOXICOLOGY LETTERS
发表日期: 2010-08-16
DOI: 10.1016/j.toxlet.2010.05.005
卷: 197, 期:2, 页:113-122
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要: Crotonaldehyde is an environment pollutant and lipid peroxidation product Crotonaldehyde produces adverse effects to humans and serves as a risk factor for human pulmonary diseases Like acrolein and 4-hydroxynonenal, crotonaldehyde seems likely to alter many cell signaling cascades, including inflammatory responses The purpose of this study was to investigate the genome-wide transcriptional responses of normal human bronchial epithelial cells exposed to crotonaldehyde Using microarrays technology, the global changes in transcriptional level were analyzed. Prior to RNA extraction, cells were exposed to crotonaldehyde at 40 or 80 mu M for 3 or 6 h Real-time quantitative polymerase chain reaction (qPCR) was performed to validate microarray data and cell cycle arrest was determined The commonly differentially regulated genes in many biological processes were dysregulated including inflammatory responses, exogenous metabolism, cell cycle, heat shock responses, and antioxidant responses Results in the present study screen out the important roles of HMOX1 in regulating other signaling cascades and ALDH1A3 in detoxifying exogenous toxicants Collectively, our study demonstrated that crotonaldehyde altered gene expression profile in the genome-wide transcriptional level in normal human bronchial epithelial cells. And many of them represented potential mechanisms of crotonaldehyde causing cytotoxicity and tissue injury in the human lung (C) 2010 Elsevier Ireland Ltd All rights reserved
关键词[WOS]: LIVER ALDEHYDE DEHYDROGENASES ;  KAPPA-B ACTIVATION ;  CIGARETTE-SMOKE ;  ALPHA,BETA-UNSATURATED ALDEHYDES ;  LIPID-PEROXIDATION ;  OXIDATIVE STRESS ;  A549 CELLS ;  TRANSCRIPTIONAL RESPONSES ;  ACROLEIN TOXICITY ;  HEME OXYGENASE-1
语种: 英语
WOS记录号: WOS:000280052200008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141864
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Zhengzhou Tobacco Res Inst CNTC, Zhengzhou 450001, Henan, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Beijing Inst Radiat Med, Dept Radiat Toxicol & Oncol, Beijing 100850, Peoples R China

Recommended Citation:
Liu, Xing-yu,Yang, Zhi-hua,Pan, Xiu-jie,et al. Gene expression profile and cytotoxicity of human bronchial epithelial cells exposed to crotonaldehyde[J]. TOXICOLOGY LETTERS,2010,197(2):113-122.
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