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题名: Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry
作者: Wang, Jian-Hua2, 3;  Quan, Chun-Shan1;  Qi, Xiao-Hui1;  Li, Xin2, 3;  Fan, Sheng-Di1
关键词: Quorum sensing ;  Signal molecules ;  Burkholderia cepacia CF-66 ;  Gas chromatography-mass spectrometry ;  Polymerase chain reaction ;  Diketopiperazines
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2010-03-01
DOI: 10.1007/s00216-009-3379-3
卷: 396, 期:5, 页:1773-1779
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Bacteria communicate with each other by a process termed "quorum sensing" (QS), and diffusible, low-molecular-weight chemicals, called signal molecules, are used as the communication languages. In cell-free Burkholderia cepacia CF-66 culture supernatants, five compounds suspected of being signal molecules were identified. The gene (cepI) related with AHLs synthesis were not detected by polymerase chain reaction (PCR) using specific primers. Gas chromatography-mass spectrometry (GC-MS) revealed that these compounds were not AHLs but the diketopiperazines (DKPs) cyclo(ProPhe), cyclo(Pro-Tyr), cyclo(Ala-Val), cyclo(Pro-Leu), and cyclo(Pro-Val), all of which were both D and L-type. Four kinds of DKPs had been isolated from other Gramnegative bacteria, but the other was a novel kind discovered in CF-66, and L-cyclo (Pro-Phe) was quantified by GC-MS. It was found that exogenous DKPs had a negative effect on the candidacidal activity of the culture supernatant extracts.
关键词[WOS]: LACTONE SIGNAL MOLECULES ;  GRAM-NEGATIVE BACTERIA ;  PSEUDOMONAS-AERUGINOSA ;  HOMOSERINE LACTONES ;  ANTIFUNGAL ACTIVITY ;  CYCLIC DIPEPTIDES ;  CROSS-TALK ;  QUORUM ;  IDENTIFICATION ;  COMMUNICATION
语种: 英语
WOS记录号: WOS:000274742900019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141878
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Nationalities Univ, Life Sci Coll, Dalian 116600, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Wang, Jian-Hua,Quan, Chun-Shan,Qi, Xiao-Hui,et al. Determination of diketopiperazines of Burkholderia cepacia CF-66 by gas chromatography-mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,396(5):1773-1779.
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