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题名: Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide
作者: Xu, Qingsong1, 2;  Ma, Pan1, 2;  Yu, Weiting1;  Tan, Chengyu3;  Liu, Hongtao1, 2;  Xiong, Chuannan1, 2;  Qiao, Ying1, 2;  Du, Yuguang1
关键词: Chitooligosaccharides ;  Human embryonic hepatocytes ;  Oxidative stress ;  Apoptosis
刊名: MARINE BIOTECHNOLOGY
发表日期: 2010-06-01
DOI: 10.1007/s10126-009-9222-1
卷: 12, 期:3, 页:292-298
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Marine & Freshwater Biology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Marine & Freshwater Biology
英文摘要: Chitooligosaccharides (COS) has many biological activities, such as antitumor activity and hepatoprotective effect. Herein, we investigated the protective effect of COS against hydrogen peroxide (H(2)O(2))-induced oxidative stress on human embryonic hepatocytes (L02 cells) and its scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical in vitro. The results showed that the lost cell viability induced by H(2)O(2) was markedly restored after 24 h pre-incubation with COS (0.1-0.4 mg/ml). This rescue effect could be related to the antioxidant property of COS, in which we showed that the radical scavenging activity of COS reached 80% at concentration of 2 mg/ml. In addition, COS could prevent cell apoptosis induced by H(2)O(2), as shown by the inhibition of the cleavage of poly (adenosine diphosphate-ribose) polymerase and increased expression of the anti-apoptotic protein Bcl-xL. Furthermore, we have utilized confocal laser microscopy to observe cellular uptake of COS, an important step for COS to exert its effects on target cells. Taken together, our findings suggested that COS could effectively protect L02 cells against oxidative stress, which might be useful in clinical setting during the treatment of oxidative stress-related liver damages.
关键词[WOS]: ANTIOXIDANT ACTIVITIES ;  IN-VITRO ;  CHITOSAN OLIGOSACCHARIDE ;  INDUCED APOPTOSIS ;  MOLECULAR-WEIGHT ;  MECHANISMS ;  PATHWAY ;  CELLS ;  MICE ;  OLIGOCHITOSAN
语种: 英语
WOS记录号: WOS:000278095400007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141879
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China

Recommended Citation:
Xu, Qingsong,Ma, Pan,Yu, Weiting,et al. Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide[J]. MARINE BIOTECHNOLOGY,2010,12(3):292-298.
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