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Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide
Xu, Qingsong1,2; Ma, Pan1,2; Yu, Weiting1; Tan, Chengyu3; Liu, Hongtao1,2; Xiong, Chuannan1,2; Qiao, Ying1,2; Du, Yuguang1
关键词Chitooligosaccharides Human Embryonic Hepatocytes Oxidative Stress Apoptosis
刊名MARINE BIOTECHNOLOGY
2010-06-01
DOI10.1007/s10126-009-9222-1
12期:3页:292-298
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]ANTIOXIDANT ACTIVITIES ; IN-VITRO ; CHITOSAN OLIGOSACCHARIDE ; INDUCED APOPTOSIS ; MOLECULAR-WEIGHT ; MECHANISMS ; PATHWAY ; CELLS ; MICE ; OLIGOCHITOSAN
英文摘要Chitooligosaccharides (COS) has many biological activities, such as antitumor activity and hepatoprotective effect. Herein, we investigated the protective effect of COS against hydrogen peroxide (H(2)O(2))-induced oxidative stress on human embryonic hepatocytes (L02 cells) and its scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical in vitro. The results showed that the lost cell viability induced by H(2)O(2) was markedly restored after 24 h pre-incubation with COS (0.1-0.4 mg/ml). This rescue effect could be related to the antioxidant property of COS, in which we showed that the radical scavenging activity of COS reached 80% at concentration of 2 mg/ml. In addition, COS could prevent cell apoptosis induced by H(2)O(2), as shown by the inhibition of the cleavage of poly (adenosine diphosphate-ribose) polymerase and increased expression of the anti-apoptotic protein Bcl-xL. Furthermore, we have utilized confocal laser microscopy to observe cellular uptake of COS, an important step for COS to exert its effects on target cells. Taken together, our findings suggested that COS could effectively protect L02 cells against oxidative stress, which might be useful in clinical setting during the treatment of oxidative stress-related liver damages.
语种英语
WOS记录号WOS:000278095400007
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141879
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
3.Dalian Fisheries Univ, Coll Marine Environm Engn, Dalian 116023, Peoples R China
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Xu, Qingsong,Ma, Pan,Yu, Weiting,et al. Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide[J]. MARINE BIOTECHNOLOGY,2010,12(3):292-298.
APA Xu, Qingsong.,Ma, Pan.,Yu, Weiting.,Tan, Chengyu.,Liu, Hongtao.,...&Du, Yuguang.(2010).Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide.MARINE BIOTECHNOLOGY,12(3),292-298.
MLA Xu, Qingsong,et al."Chitooligosaccharides Protect Human Embryonic Hepatocytes Against Oxidative Stress Induced by Hydrogen Peroxide".MARINE BIOTECHNOLOGY 12.3(2010):292-298.
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