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Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device
Liu, Tingjiao1,2; Lin, Bingcheng1; Qin, Jianhua1
刊名LAB ON A CHIP
2010
DOI10.1039/c000022a
10期:13页:1671-1677
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
关键词[WOS]CANCER-CELLS ; STROMAL FIBROBLASTS ; HOMOTYPIC AGGREGATION ; IN-VIVO ; MYOFIBROBLASTS ; ANGIOGENESIS ; EXPRESSION ; CULTURE ; PROGRESSION ; MATRIX
英文摘要Carcinoma-associated fibroblasts (CAFs) are a key determinant in malignant progression of cancer and represent an important target for cancer therapies. In this work, we present a microfluidic-based 3D co-culture device to reconstruct an in vitro tumor microenvironment and firstly investigate the effect of CAFs on cancer cell invasion in 3D matrix. This device is composed of six co-culture units, which enable parallel co-culture assays to be run in the presence of 3D extracellular matrix. Salivary gland adenoid cystic carcinoma (ACC) cells and CAFs embedded in matrix were co-cultured without direct contact on the device. Communication between ACC cells and CAFs could be established via medium diffused in matrix. It was observed that CAFs promoted ACC cell invasion in 3D matrix in a spheroid fashion, indicating that CAFs play a critical role in cancer invasion. We further demonstrated the effect of MMP inhibitor as an agent against CAF-promoted cancer invasion. This co-culture device reproducibly reflected the in vivo growth and invasion pattern of ACC and recreated the stroma-regulated ACC invasion. Thus, it provides a suitable platform for elucidating the mechanism of CAF-regulated cancer invasion and discovering anti-invasion drugs in a well defined 3D environment.
语种英语
WOS记录号WOS:000278824600004
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141885
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Beijing 100864, Peoples R China
2.Dalian Med Univ, Sect Oral Pathol, Coll Stomatol, Dalian, Peoples R China
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Liu, Tingjiao,Lin, Bingcheng,Qin, Jianhua. Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device[J]. LAB ON A CHIP,2010,10(13):1671-1677.
APA Liu, Tingjiao,Lin, Bingcheng,&Qin, Jianhua.(2010).Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device.LAB ON A CHIP,10(13),1671-1677.
MLA Liu, Tingjiao,et al."Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device".LAB ON A CHIP 10.13(2010):1671-1677.
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