DICP OpenIR
Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device
Liu, Tingjiao1,2; Lin, Bingcheng1; Qin, Jianhua1
Source PublicationLAB ON A CHIP
2010
DOI10.1039/c000022a
Volume10Issue:13Pages:1671-1677
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics
WOS KeywordCANCER-CELLS ; STROMAL FIBROBLASTS ; HOMOTYPIC AGGREGATION ; IN-VIVO ; MYOFIBROBLASTS ; ANGIOGENESIS ; EXPRESSION ; CULTURE ; PROGRESSION ; MATRIX
AbstractCarcinoma-associated fibroblasts (CAFs) are a key determinant in malignant progression of cancer and represent an important target for cancer therapies. In this work, we present a microfluidic-based 3D co-culture device to reconstruct an in vitro tumor microenvironment and firstly investigate the effect of CAFs on cancer cell invasion in 3D matrix. This device is composed of six co-culture units, which enable parallel co-culture assays to be run in the presence of 3D extracellular matrix. Salivary gland adenoid cystic carcinoma (ACC) cells and CAFs embedded in matrix were co-cultured without direct contact on the device. Communication between ACC cells and CAFs could be established via medium diffused in matrix. It was observed that CAFs promoted ACC cell invasion in 3D matrix in a spheroid fashion, indicating that CAFs play a critical role in cancer invasion. We further demonstrated the effect of MMP inhibitor as an agent against CAF-promoted cancer invasion. This co-culture device reproducibly reflected the in vivo growth and invasion pattern of ACC and recreated the stroma-regulated ACC invasion. Thus, it provides a suitable platform for elucidating the mechanism of CAF-regulated cancer invasion and discovering anti-invasion drugs in a well defined 3D environment.
Language英语
WOS IDWOS:000278824600004
Citation statistics
Cited Times:84[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141885
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Beijing 100864, Peoples R China
2.Dalian Med Univ, Sect Oral Pathol, Coll Stomatol, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Liu, Tingjiao,Lin, Bingcheng,Qin, Jianhua. Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device[J]. LAB ON A CHIP,2010,10(13):1671-1677.
APA Liu, Tingjiao,Lin, Bingcheng,&Qin, Jianhua.(2010).Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device.LAB ON A CHIP,10(13),1671-1677.
MLA Liu, Tingjiao,et al."Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device".LAB ON A CHIP 10.13(2010):1671-1677.
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