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题名: Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device
作者: Liu, Tingjiao1, 2;  Lin, Bingcheng1;  Qin, Jianhua1
刊名: LAB ON A CHIP
发表日期: 2010
DOI: 10.1039/c000022a
卷: 10, 期:13, 页:1671-1677
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Multidisciplinary ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Science & Technology - Other Topics
英文摘要: Carcinoma-associated fibroblasts (CAFs) are a key determinant in malignant progression of cancer and represent an important target for cancer therapies. In this work, we present a microfluidic-based 3D co-culture device to reconstruct an in vitro tumor microenvironment and firstly investigate the effect of CAFs on cancer cell invasion in 3D matrix. This device is composed of six co-culture units, which enable parallel co-culture assays to be run in the presence of 3D extracellular matrix. Salivary gland adenoid cystic carcinoma (ACC) cells and CAFs embedded in matrix were co-cultured without direct contact on the device. Communication between ACC cells and CAFs could be established via medium diffused in matrix. It was observed that CAFs promoted ACC cell invasion in 3D matrix in a spheroid fashion, indicating that CAFs play a critical role in cancer invasion. We further demonstrated the effect of MMP inhibitor as an agent against CAF-promoted cancer invasion. This co-culture device reproducibly reflected the in vivo growth and invasion pattern of ACC and recreated the stroma-regulated ACC invasion. Thus, it provides a suitable platform for elucidating the mechanism of CAF-regulated cancer invasion and discovering anti-invasion drugs in a well defined 3D environment.
关键词[WOS]: CANCER-CELLS ;  STROMAL FIBROBLASTS ;  HOMOTYPIC AGGREGATION ;  IN-VIVO ;  MYOFIBROBLASTS ;  ANGIOGENESIS ;  EXPRESSION ;  CULTURE ;  PROGRESSION ;  MATRIX
语种: 英语
WOS记录号: WOS:000278824600004
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141885
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Beijing 100864, Peoples R China
2.Dalian Med Univ, Sect Oral Pathol, Coll Stomatol, Dalian, Peoples R China

Recommended Citation:
Liu, Tingjiao,Lin, Bingcheng,Qin, Jianhua. Carcinoma-associated fibroblasts promoted tumor spheroid invasion on a microfluidic 3D co-culture device[J]. LAB ON A CHIP,2010,10(13):1671-1677.
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