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Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry
Gu, Qun1,2; David, Frank1,3; Lynen, Frederic1; Rumpel, Klaus4; Xu, Guowang2; De Vos, Paul5; Sandra, Pat1,3
KeywordGc x Gc Flow Modulation Bacterial Fatty Acid Methyl Esters Parallel Detection Mass Spectrometry
Source PublicationJOURNAL OF CHROMATOGRAPHY A
2010-06-25
DOI10.1016/j.chroma.2010.04.057
Volume1217Issue:26Pages:4448-4453
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMASS-SPECTROMETRY ; CHEMICAL-IONIZATION ; ELECTRON-IMPACT ; 2-D GC ; SINGLE ; IDENTIFICATION ; SYSTEM ; DIFFERENTIATION ; SEPARATION ; INTERFACE
AbstractComprehensive two-dimensional gas chromatography (GC x GC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GC x GC using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia LMG 958(T) by using parallel flame ionization detector (FID)/mass spectrometry (MS). The results are compared to data obtained using a thermal modulated GC x GC system. The data show that flow modulated GC x GC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria. (C) 2010 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000278920900026
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141888
Collection中国科学院大连化学物理研究所
Affiliation1.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Res Inst Chromatog, B-8500 Kortrijk, Belgium
4.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England
5.Univ Ghent, Microbiol Lab, Dept Biochem & Microbiol, B-9000 Ghent, Belgium
Recommended Citation
GB/T 7714
Gu, Qun,David, Frank,Lynen, Frederic,et al. Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(26):4448-4453.
APA Gu, Qun.,David, Frank.,Lynen, Frederic.,Rumpel, Klaus.,Xu, Guowang.,...&Sandra, Pat.(2010).Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1217(26),4448-4453.
MLA Gu, Qun,et al."Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1217.26(2010):4448-4453.
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