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Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry
Gu, Qun1,2; David, Frank1,3; Lynen, Frederic1; Rumpel, Klaus4; Xu, Guowang2; De Vos, Paul5; Sandra, Pat1,3
关键词Gc x Gc Flow Modulation Bacterial Fatty Acid Methyl Esters Parallel Detection Mass Spectrometry
刊名JOURNAL OF CHROMATOGRAPHY A
2010-06-25
DOI10.1016/j.chroma.2010.04.057
1217期:26页:4448-4453
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]MASS-SPECTROMETRY ; CHEMICAL-IONIZATION ; ELECTRON-IMPACT ; 2-D GC ; SINGLE ; IDENTIFICATION ; SYSTEM ; DIFFERENTIATION ; SEPARATION ; INTERFACE
英文摘要Comprehensive two-dimensional gas chromatography (GC x GC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GC x GC using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia LMG 958(T) by using parallel flame ionization detector (FID)/mass spectrometry (MS). The results are compared to data obtained using a thermal modulated GC x GC system. The data show that flow modulated GC x GC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria. (C) 2010 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000278920900026
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141888
专题中国科学院大连化学物理研究所
作者单位1.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Res Inst Chromatog, B-8500 Kortrijk, Belgium
4.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England
5.Univ Ghent, Microbiol Lab, Dept Biochem & Microbiol, B-9000 Ghent, Belgium
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GB/T 7714
Gu, Qun,David, Frank,Lynen, Frederic,et al. Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(26):4448-4453.
APA Gu, Qun.,David, Frank.,Lynen, Frederic.,Rumpel, Klaus.,Xu, Guowang.,...&Sandra, Pat.(2010).Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry.JOURNAL OF CHROMATOGRAPHY A,1217(26),4448-4453.
MLA Gu, Qun,et al."Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry".JOURNAL OF CHROMATOGRAPHY A 1217.26(2010):4448-4453.
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