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题名: Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry
作者: Gu, Qun1, 2;  David, Frank1, 3;  Lynen, Frederic1;  Rumpel, Klaus4;  Xu, Guowang2;  De Vos, Paul5;  Sandra, Pat1, 3
关键词: GC x GC ;  Flow modulation ;  Bacterial fatty acid methyl esters ;  Parallel detection ;  Mass spectrometry
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2010-06-25
DOI: 10.1016/j.chroma.2010.04.057
卷: 1217, 期:26, 页:4448-4453
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Comprehensive two-dimensional gas chromatography (GC x GC) offers an interesting tool for profiling bacterial fatty acids. Flow modulated GC x GC using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. The method was tested on bacterial fatty acid methyl esters (BAMEs) from Stenotrophomonas maltophilia LMG 958(T) by using parallel flame ionization detector (FID)/mass spectrometry (MS). The results are compared to data obtained using a thermal modulated GC x GC system. The data show that flow modulated GC x GC-FID/MS method can be applied in a routine environment and offers interesting perspectives for chemotaxonomy of bacteria. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: MASS-SPECTROMETRY ;  CHEMICAL-IONIZATION ;  ELECTRON-IMPACT ;  2-D GC ;  SINGLE ;  IDENTIFICATION ;  SYSTEM ;  DIFFERENTIATION ;  SEPARATION ;  INTERFACE
语种: 英语
WOS记录号: WOS:000278920900026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141888
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Res Inst Chromatog, B-8500 Kortrijk, Belgium
4.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England
5.Univ Ghent, Microbiol Lab, Dept Biochem & Microbiol, B-9000 Ghent, Belgium

Recommended Citation:
Gu, Qun,David, Frank,Lynen, Frederic,et al. Analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(26):4448-4453.
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