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题名: Molecular Cloning and Characterization of a Brassica napus L. MAP Kinase Involved in Oligochitosan-Induced Defense Signaling
作者: Yin, Heng1;  Zhao, Xiaoming1;  Bai, Xuefang1;  Du, Yuguang1
关键词: Oligochitosan ;  Brassica napus ;  BnOIPK ;  Plant defense ;  Signal transduction
刊名: PLANT MOLECULAR BIOLOGY REPORTER
发表日期: 2010-06-01
DOI: 10.1007/s11105-009-0152-x
卷: 28, 期:2, 页:292-301
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemical Research Methods ;  Plant Sciences
研究领域[WOS]: Biochemistry & Molecular Biology ;  Plant Sciences
英文摘要: Oligochitosan is a potent plant defense elicitor. In this paper, a novel Brassica napus mitogen-activated protein kinase (MAPK) gene induced by oligochitosan was isolated by rapid amplification of cDNA ends technique and designated as BnOIPK (oligochitosan-induced protein kinase (OIPK)). BnOIPK, with high sequence similarity to previously reported plant MAPK genes, encodes a 373-amino acid protein and belongs to the B subgroup of plant MAPK. Bioinformatics analysis showed BnOIPK contains one phosphorylation motif (TEY) and a conserved common docking domain in its C-terminal extension. With a constitutive expression in seedling leaves, BnOIPK is further upregulated upon treatment with plant hormone jasmonic acid (JA), but did not markedly respond to salicylic acid and abscisic acid. Activation of BnOIPK transcripts induced by oligochitosan depending on nitric oxide (NO) and hydrogen peroxide (H(2)O(2)) was identified by using NO, H(2)O(2), and their scavenger, respectively. Polyclonal antibody BOK was prepared by using a 69-kD GST-BnOIPK fusion protein which was produced from pGEX-4T-1 vector. Western-blot assays showed BnOIPK could be induced by oligochitosan and JA at a short time. All these results suggest that BnOIPK might be a key node of JA-mediated defense signaling and act on the downstream of NO and H(2)O(2).
关键词[WOS]: ACTIVATED PROTEIN-KINASES ;  ENVIRONMENTAL-STRESSES ;  ABSCISIC-ACID ;  CHITOSAN ;  ARABIDOPSIS ;  RESISTANCE ;  GENE ;  PATHWAY ;  RICE ;  RESPONSES
语种: 英语
WOS记录号: WOS:000274961000012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141919
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dept Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Yin, Heng,Zhao, Xiaoming,Bai, Xuefang,et al. Molecular Cloning and Characterization of a Brassica napus L. MAP Kinase Involved in Oligochitosan-Induced Defense Signaling[J]. PLANT MOLECULAR BIOLOGY REPORTER,2010,28(2):292-301.
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