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题名: Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry
作者: Wang, Chen1, 2, 3;  Wang, Shuowen2, 3;  Fan, Guorong2, 3;  Zou, Hanfa1
关键词: Comprehensive two-dimensional biochromatography ;  Corydalis yanhusuo WT Wang ;  Human serum albumin column ;  Traditional Chinese medicine ;  Formalin test
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2010-03-01
DOI: 10.1007/s00216-009-3409-1
卷: 396, 期:5, 页:1731-1740
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Formalin-induced pain models were used in rats to evaluate the antinociceptive effect of the total alkaloids of Corydalis yanhusuo (TAC). The results indicated that formalin-evoked spontaneous nociceptive responses (licking behavior) could be inhibited significantly by giving (intragingival) TAC at a single dose of 150 mg/kg. Subsequently, an online comprehensive two-dimensional biochromatography method with a silica-bonded human serum albumin (HSA) column in the first dimension and a monolithic ODS column in the second was developed. The absorbed bioactive components were screened by comparing and contrasting the components detected in the plasma and striatum with those in TAC. More than 100 compounds were separated and detected in the TAC, among which 13 compounds were identified. About 40 compounds (seven compounds identified) were absorbed into the plasma with appropriate concentrations and about 20 compounds (four compounds identified) passed through the blood-brain barrier into the striatum. Of interest, four compounds (protopine, glaucine, tetrahydropalmatine, and corydaline) which were reported to possess profound antinociceptive effects exhibited high concentrations in the striatum. This may result from their synergistic effects in regulating the formalin-induced nociception. The results indicated that the comprehensive two-dimensional biochromatography method developed is capable of screening the bioactive components in Corydalis yanhusuo and providing valuable information for understanding the mechanisms by which Corydalis yanhusuo alleviates nociception.
关键词[WOS]: BIOLOGICAL FINGERPRINTING ANALYSIS ;  TRADITIONAL CHINESE MEDICINES ;  LONGDAN-XIEGAN-DECOCTION ;  DOPAMINE-RECEPTORS ;  STATIONARY-PHASE ;  D-1 RECEPTOR ;  MODELS ;  IDENTIFICATION ;  COLUMN ;  MICE
语种: 英语
WOS记录号: WOS:000274742900014
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141922
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Dept Pharmaceut Anal, Sch Pharm, Shanghai 200433, Peoples R China
3.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China

Recommended Citation:
Wang, Chen,Wang, Shuowen,Fan, Guorong,et al. Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,396(5):1731-1740.
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