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Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry
Wang, Chen1,2,3; Wang, Shuowen2,3; Fan, Guorong2,3; Zou, Hanfa1
关键词Comprehensive Two-dimensional Biochromatography Corydalis Yanhusuo Wt Wang Human Serum Albumin Column Traditional Chinese Medicine Formalin Test
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2010-03-01
DOI10.1007/s00216-009-3409-1
396期:5页:1731-1740
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BIOLOGICAL FINGERPRINTING ANALYSIS ; TRADITIONAL CHINESE MEDICINES ; LONGDAN-XIEGAN-DECOCTION ; DOPAMINE-RECEPTORS ; STATIONARY-PHASE ; D-1 RECEPTOR ; MODELS ; IDENTIFICATION ; COLUMN ; MICE
英文摘要Formalin-induced pain models were used in rats to evaluate the antinociceptive effect of the total alkaloids of Corydalis yanhusuo (TAC). The results indicated that formalin-evoked spontaneous nociceptive responses (licking behavior) could be inhibited significantly by giving (intragingival) TAC at a single dose of 150 mg/kg. Subsequently, an online comprehensive two-dimensional biochromatography method with a silica-bonded human serum albumin (HSA) column in the first dimension and a monolithic ODS column in the second was developed. The absorbed bioactive components were screened by comparing and contrasting the components detected in the plasma and striatum with those in TAC. More than 100 compounds were separated and detected in the TAC, among which 13 compounds were identified. About 40 compounds (seven compounds identified) were absorbed into the plasma with appropriate concentrations and about 20 compounds (four compounds identified) passed through the blood-brain barrier into the striatum. Of interest, four compounds (protopine, glaucine, tetrahydropalmatine, and corydaline) which were reported to possess profound antinociceptive effects exhibited high concentrations in the striatum. This may result from their synergistic effects in regulating the formalin-induced nociception. The results indicated that the comprehensive two-dimensional biochromatography method developed is capable of screening the bioactive components in Corydalis yanhusuo and providing valuable information for understanding the mechanisms by which Corydalis yanhusuo alleviates nociception.
语种英语
WOS记录号WOS:000274742900014
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141922
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Dept Pharmaceut Anal, Sch Pharm, Shanghai 200433, Peoples R China
3.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
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GB/T 7714
Wang, Chen,Wang, Shuowen,Fan, Guorong,et al. Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,396(5):1731-1740.
APA Wang, Chen,Wang, Shuowen,Fan, Guorong,&Zou, Hanfa.(2010).Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,396(5),1731-1740.
MLA Wang, Chen,et al."Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 396.5(2010):1731-1740.
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