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Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry
Wang, Chen1,2,3; Wang, Shuowen2,3; Fan, Guorong2,3; Zou, Hanfa1
KeywordComprehensive Two-dimensional Biochromatography Corydalis Yanhusuo Wt Wang Human Serum Albumin Column Traditional Chinese Medicine Formalin Test
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2010-03-01
DOI10.1007/s00216-009-3409-1
Volume396Issue:5Pages:1731-1740
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordBIOLOGICAL FINGERPRINTING ANALYSIS ; TRADITIONAL CHINESE MEDICINES ; LONGDAN-XIEGAN-DECOCTION ; DOPAMINE-RECEPTORS ; STATIONARY-PHASE ; D-1 RECEPTOR ; MODELS ; IDENTIFICATION ; COLUMN ; MICE
AbstractFormalin-induced pain models were used in rats to evaluate the antinociceptive effect of the total alkaloids of Corydalis yanhusuo (TAC). The results indicated that formalin-evoked spontaneous nociceptive responses (licking behavior) could be inhibited significantly by giving (intragingival) TAC at a single dose of 150 mg/kg. Subsequently, an online comprehensive two-dimensional biochromatography method with a silica-bonded human serum albumin (HSA) column in the first dimension and a monolithic ODS column in the second was developed. The absorbed bioactive components were screened by comparing and contrasting the components detected in the plasma and striatum with those in TAC. More than 100 compounds were separated and detected in the TAC, among which 13 compounds were identified. About 40 compounds (seven compounds identified) were absorbed into the plasma with appropriate concentrations and about 20 compounds (four compounds identified) passed through the blood-brain barrier into the striatum. Of interest, four compounds (protopine, glaucine, tetrahydropalmatine, and corydaline) which were reported to possess profound antinociceptive effects exhibited high concentrations in the striatum. This may result from their synergistic effects in regulating the formalin-induced nociception. The results indicated that the comprehensive two-dimensional biochromatography method developed is capable of screening the bioactive components in Corydalis yanhusuo and providing valuable information for understanding the mechanisms by which Corydalis yanhusuo alleviates nociception.
Language英语
WOS IDWOS:000274742900014
Citation statistics
Cited Times:54[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141922
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Second Mil Med Univ, Dept Pharmaceut Anal, Sch Pharm, Shanghai 200433, Peoples R China
3.Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
Recommended Citation
GB/T 7714
Wang, Chen,Wang, Shuowen,Fan, Guorong,et al. Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2010,396(5):1731-1740.
APA Wang, Chen,Wang, Shuowen,Fan, Guorong,&Zou, Hanfa.(2010).Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,396(5),1731-1740.
MLA Wang, Chen,et al."Screening of antinociceptive components in Corydalis yanhusuo WT Wang by comprehensive two-dimensional liquid chromatography/tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 396.5(2010):1731-1740.
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