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题名: Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column
作者: Li-Yuan, Zhang1, 2;  Hui, Wang1, 2;  Zhen, Liang1;  Yi-Chu, Shan1;  Li-Hua, Zhang1;  Yu-Kui, Zhang1
关键词: Immobilized metal affinity chromatography ;  Phosphopeptides ;  Selective enrichment ;  Monolith ;  Iron
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2010-05-01
卷: 38, 期:5, 页:659-662
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A novel method to prepare monolithic Fe(3+) immobilized affinity chromatography column was developed, which included in situ polymerization of N-acryloxysuccinimide and ethylene dimethacrylate, nitilotriacetic acid activation, and Fe(3+) immobilization. The monolithic column showed high selectivity, high binding capacity, good recovery and good reproducibility for the enrichment of phosphopeptides. Such a column was successfully applied to the enrichment of phosphopeptides from the nonfat milk protein digests. All results demonstrate the promising of such a column in phosphoproteome analysis.
关键词[WOS]: PHOSPHOPEPTIDE ENRICHMENT ;  PHOSPHOPROTEOME ANALYSIS ;  PEPTIDES ;  PROTEINS
语种: 英语
WOS记录号: WOS:000278208600010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141941
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Li-Yuan, Zhang,Hui, Wang,Zhen, Liang,et al. Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(5):659-662.
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