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Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column
Li-Yuan, Zhang1,2; Hui, Wang1,2; Zhen, Liang1; Yi-Chu, Shan1; Li-Hua, Zhang1; Yu-Kui, Zhang1
关键词Immobilized Metal Affinity Chromatography Phosphopeptides Selective Enrichment Monolith Iron
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2010-05-01
38期:5页:659-662
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PHOSPHOPEPTIDE ENRICHMENT ; PHOSPHOPROTEOME ANALYSIS ; PEPTIDES ; PROTEINS
英文摘要A novel method to prepare monolithic Fe(3+) immobilized affinity chromatography column was developed, which included in situ polymerization of N-acryloxysuccinimide and ethylene dimethacrylate, nitilotriacetic acid activation, and Fe(3+) immobilization. The monolithic column showed high selectivity, high binding capacity, good recovery and good reproducibility for the enrichment of phosphopeptides. Such a column was successfully applied to the enrichment of phosphopeptides from the nonfat milk protein digests. All results demonstrate the promising of such a column in phosphoproteome analysis.
语种英语
WOS记录号WOS:000278208600010
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141941
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R & A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
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Li-Yuan, Zhang,Hui, Wang,Zhen, Liang,et al. Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(5):659-662.
APA Li-Yuan, Zhang,Hui, Wang,Zhen, Liang,Yi-Chu, Shan,Li-Hua, Zhang,&Yu-Kui, Zhang.(2010).Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38(5),659-662.
MLA Li-Yuan, Zhang,et al."Preparation and Evaluation of Novel Iron Immobilized Metal Affinity Chromatography Monolith Column".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 38.5(2010):659-662.
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