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题名: Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system
作者: Yang, Qin1;  Shi, Xianzhe1;  Wang, Yuan1;  Wang, Wenzhao1;  He, Hongbin1;  Lu, Xin1;  Xu, Guowang1
关键词: Column switching ;  Hydrophilic interaction chromatography ;  Lung cancer ;  Metabonomics ;  Two-dimensional LC
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2010-05-01
DOI: 10.1002/jssc.200900798
卷: 33, 期:10, 页:1495-1503
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Lung cancer is one of the most common and lethal cancers in the world. In this study, a home-devised hydrophilic interaction chromatography/RPLC-MS (HILIC/RPLC-MS) system was developed to study the urinary metabonomics of lung cancer patients. This system combined the orthogonal selectivity of HILIC and RPLC and could chromatographically reveal more comprehensive information of the urinary metabolites. Within a total analysis time of 50 min, we detected 577 polar metabolite ions on the first HILIC column and 261 apolar ones on the second RPLC column. In addition, an orthogonal signal correction partial least-squares discriminant analysis model was constructed to characterize differences between health and lung cancer cases. Eleven potential biomarkers, ten from HILIC column and one from the second RP column, were identified and all of these biomarkers were found upregulated in lung cancer patients. Overall, the results indicated that the developed HILIC/RPLC-MS system is a promising tool for metabonomic studies in revealing more information of highly complex samples.
关键词[WOS]: LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY ;  MASS-SPECTROMETRY ;  MAGNETIC-RESONANCE ;  RAT URINE ;  TOF-MS ;  PHASE ;  HPLC ;  ACID ;  IDENTIFICATION ;  SPECTROSCOPY
语种: 英语
WOS记录号: WOS:000278572000017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141947
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Qin,Shi, Xianzhe,Wang, Yuan,et al. Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(10):1495-1503.
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