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Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system
Yang, Qin; Shi, Xianzhe; Wang, Yuan; Wang, Wenzhao; He, Hongbin; Lu, Xin; Xu, Guowang
关键词Column Switching Hydrophilic Interaction Chromatography Lung Cancer Metabonomics Two-dimensional Lc
刊名JOURNAL OF SEPARATION SCIENCE
2010-05-01
DOI10.1002/jssc.200900798
33期:10页:1495-1503
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY ; MASS-SPECTROMETRY ; MAGNETIC-RESONANCE ; RAT URINE ; TOF-MS ; PHASE ; HPLC ; ACID ; IDENTIFICATION ; SPECTROSCOPY
英文摘要Lung cancer is one of the most common and lethal cancers in the world. In this study, a home-devised hydrophilic interaction chromatography/RPLC-MS (HILIC/RPLC-MS) system was developed to study the urinary metabonomics of lung cancer patients. This system combined the orthogonal selectivity of HILIC and RPLC and could chromatographically reveal more comprehensive information of the urinary metabolites. Within a total analysis time of 50 min, we detected 577 polar metabolite ions on the first HILIC column and 261 apolar ones on the second RPLC column. In addition, an orthogonal signal correction partial least-squares discriminant analysis model was constructed to characterize differences between health and lung cancer cases. Eleven potential biomarkers, ten from HILIC column and one from the second RP column, were identified and all of these biomarkers were found upregulated in lung cancer patients. Overall, the results indicated that the developed HILIC/RPLC-MS system is a promising tool for metabonomic studies in revealing more information of highly complex samples.
语种英语
WOS记录号WOS:000278572000017
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被引频次:57[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/141947
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Yang, Qin,Shi, Xianzhe,Wang, Yuan,et al. Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(10):1495-1503.
APA Yang, Qin.,Shi, Xianzhe.,Wang, Yuan.,Wang, Wenzhao.,He, Hongbin.,...&Xu, Guowang.(2010).Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system.JOURNAL OF SEPARATION SCIENCE,33(10),1495-1503.
MLA Yang, Qin,et al."Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system".JOURNAL OF SEPARATION SCIENCE 33.10(2010):1495-1503.
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