DICP OpenIR
Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system
Yang, Qin; Shi, Xianzhe; Wang, Yuan; Wang, Wenzhao; He, Hongbin; Lu, Xin; Xu, Guowang
KeywordColumn Switching Hydrophilic Interaction Chromatography Lung Cancer Metabonomics Two-dimensional Lc
Source PublicationJOURNAL OF SEPARATION SCIENCE
2010-05-01
DOI10.1002/jssc.200900798
Volume33Issue:10Pages:1495-1503
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordLIQUID CHROMATOGRAPHY/MASS SPECTROMETRY ; MASS-SPECTROMETRY ; MAGNETIC-RESONANCE ; RAT URINE ; TOF-MS ; PHASE ; HPLC ; ACID ; IDENTIFICATION ; SPECTROSCOPY
AbstractLung cancer is one of the most common and lethal cancers in the world. In this study, a home-devised hydrophilic interaction chromatography/RPLC-MS (HILIC/RPLC-MS) system was developed to study the urinary metabonomics of lung cancer patients. This system combined the orthogonal selectivity of HILIC and RPLC and could chromatographically reveal more comprehensive information of the urinary metabolites. Within a total analysis time of 50 min, we detected 577 polar metabolite ions on the first HILIC column and 261 apolar ones on the second RPLC column. In addition, an orthogonal signal correction partial least-squares discriminant analysis model was constructed to characterize differences between health and lung cancer cases. Eleven potential biomarkers, ten from HILIC column and one from the second RP column, were identified and all of these biomarkers were found upregulated in lung cancer patients. Overall, the results indicated that the developed HILIC/RPLC-MS system is a promising tool for metabonomic studies in revealing more information of highly complex samples.
Language英语
WOS IDWOS:000278572000017
Citation statistics
Cited Times:60[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141947
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yang, Qin,Shi, Xianzhe,Wang, Yuan,et al. Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system[J]. JOURNAL OF SEPARATION SCIENCE,2010,33(10):1495-1503.
APA Yang, Qin.,Shi, Xianzhe.,Wang, Yuan.,Wang, Wenzhao.,He, Hongbin.,...&Xu, Guowang.(2010).Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system.JOURNAL OF SEPARATION SCIENCE,33(10),1495-1503.
MLA Yang, Qin,et al."Urinary metabonomic study of lung cancer by a fully automatic hyphenated hydrophilic interaction/RPLC-MS system".JOURNAL OF SEPARATION SCIENCE 33.10(2010):1495-1503.
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