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题名: Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity
作者: Li, Yun1, 2;  Tolley, H. Dennis3;  Lee, Milton L.1
关键词: Monolithic columns ;  Liquid chromatography ;  Capillary columns ;  Size exclusion chromatography ;  Pore size distribution ;  Porosity ;  Biocompatible ;  Polyethylene glycol diacrylate ;  Brij ;  Proteins ;  Peptides
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2010-12-24
DOI: 10.1016/j.chroma.2010.10.067
卷: 1217, 期:52, 页:8181-8185
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Biocompatible poly(ethylene glycol methyl ether acrylate-co-polyethylene glycol diacrylate) monoliths were prepared for size exclusion chromatography (SEC) of proteins in the capillary format using Brij 58P in a mixture of hexanes and dodecanol as porogens. The monolithic columns provided size separation of four proteins in 20 mM sodium phosphate buffer (pH 7.0) containing 0.15 M NaCl, and there was a linear relationship between the retention times and the logarithmic values of the molecular weights. Compared to SEC monoliths previously synthesized using a triblock copolymer of polyethylene oxide and polypropylene oxide, an increase in mesoporosity was confirmed by inverse size exclusion chromatography. As a result, improved protein separation in the high molecular weight range and reduced column back-pressure were observed. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: HYDROPHOBIC INTERACTION CHROMATOGRAPHY ;  HYDROPHILIC INTERACTION CHROMATOGRAPHY ;  LIQUID-CHROMATOGRAPHY ;  PEPTIDES ;  ROD
语种: 英语
WOS记录号: WOS:000285899400013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/141969
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China
3.Brigham Young Univ, Dept Stat, Provo, UT 84602 USA

Recommended Citation:
Li, Yun,Tolley, H. Dennis,Lee, Milton L.. Size-exclusion separation of proteins using a biocompatible polymeric monolithic capillary column with mesoporosity[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(52):8181-8185.
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