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Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device
Ma, Huipeng1,2; Liu, Tingjiao1,3; Qin, Jianhua1; Lin, Bingcheng1
KeywordCo-culture Microfluidic Migration Transdifferentiation
Source PublicationELECTROPHORESIS
2010-05-01
DOI10.1002/elps.200900776
Volume31Issue:10Pages:1599-1605
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordMYOFIBROBLAST DIFFERENTIATION ; CANCER ; MICROENVIRONMENT ; INVASION ; STROMA ; CHIP ; ANGIOGENESIS ; SEPARATION ; TISSUE
AbstractFibroblasts and tumor cells have been involved in the process of cancer development, progression and therapy. Here, we present a simple microfluidic device which enables to study the interaction between fibroblasts and tumor cells by indirect contact co-culture. The device is composed of multiple cell culture chambers which are connected by a parallel of cell migration regions, and it enables to realize different types of cells to communicate each other on the single device. In this work, human embryonic lung fibroblasts cells were observed to exhibit obvious migration towards tumor cells instead of normal epithelial cells on the co-culture device. Moreover, transdifferentiation of human embryonic lung fibroblast cells was recognized by the specific expression of a-smooth musle actin, indicating the effect of tumor cells on the behavior of fibroblasts. Furthermore, multiple types of cell co-culture can be demonstrated on the single device which enables to mimic the complicated microenviroment in vivo. The device is simple and easy to operate, which enables to realize real-time observation of cell migration after external stimulus. This microfluidic device allows for the characterization of various cellular events on a single device sequentially, faciliating the better understanding of interaction between heterotypic cells in a more complex microenvironment.
Language英语
WOS IDWOS:000278650500001
Citation statistics
Cited Times:47[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/141978
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Dalian Med Univ, Coll Stomatol, Sect Oral Pathol, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Ma, Huipeng,Liu, Tingjiao,Qin, Jianhua,et al. Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device[J]. ELECTROPHORESIS,2010,31(10):1599-1605.
APA Ma, Huipeng,Liu, Tingjiao,Qin, Jianhua,&Lin, Bingcheng.(2010).Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device.ELECTROPHORESIS,31(10),1599-1605.
MLA Ma, Huipeng,et al."Characterization of the interaction between fibroblasts and tumor cells on a microfluidic co-culture device".ELECTROPHORESIS 31.10(2010):1599-1605.
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