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Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect
Tan, Haidong2; Fu, Li3; Seno, Masaharu1
关键词Sepiolite Carbon Nanotube Plasmid Transformation Nanomaterials Yoshida Effect
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2010-12-01
DOI10.3390/ijms11124962
11期:12页:4962-4973
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]ESCHERICHIA-COLI-CELLS ; CHRYSOTILE ASBESTOS ; CARBON NANOTUBES ; DNA ; FIBERS
英文摘要With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.
语种英语
WOS记录号WOS:000285708000013
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被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142074
专题中国科学院大连化学物理研究所
作者单位1.Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn Sci, Okayama 7008530, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
3.Tianjin Med Univ, Canc Hosp, State Key Lab Breast Canc Res, Dept Breast Canc Pathol & Res Lab, Tianjin 300060, Peoples R China
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Tan, Haidong,Fu, Li,Seno, Masaharu. Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2010,11(12):4962-4973.
APA Tan, Haidong,Fu, Li,&Seno, Masaharu.(2010).Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,11(12),4962-4973.
MLA Tan, Haidong,et al."Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 11.12(2010):4962-4973.
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