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题名: Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect
作者: Tan, Haidong2;  Fu, Li3;  Seno, Masaharu1
关键词: sepiolite ;  carbon nanotube ;  plasmid transformation ;  nanomaterials ;  Yoshida effect
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2010-12-01
DOI: 10.3390/ijms11124962
卷: 11, 期:12, 页:4962-4973
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: With the help of sepiolite, a unique method for transforming DNA into bacteria, based on the Yoshida effect, has been developed recently. However, we confronted many problems when this newest method was tried. Only a few transformants could be obtained even when 100 ng of plasmid pET15b was used, and a successful result seemed difficult to repeat. To address this problem, we optimized the operating method and could achieve about 15,000 transformants using the same amount of plasmid, which could match the efficiency gained using the calcium chloride transformation method. Meanwhile, the results could also be reproduced well. In the same way, carbon nanotubes were used to attain more than 15,000 transformants in the same situation. Therefore, the transformation method could be extended to other nanomaterials. Meanwhile, compared with the mechanism previously reported, we verified quite a different principle for the mechanism responsible for such a transformation. In sum, this unique transformation can be developed to become the third widely-used transformation method in laboratories in addition to the chemical method and electroporation.
关键词[WOS]: ESCHERICHIA-COLI-CELLS ;  CHRYSOTILE ASBESTOS ;  CARBON NANOTUBES ;  DNA ;  FIBERS
语种: 英语
WOS记录号: WOS:000285708000013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142074
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Okayama Univ, Grad Sch Nat Sci & Technol, Dept Med & Bioengn Sci, Okayama 7008530, Japan
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
3.Tianjin Med Univ, Canc Hosp, State Key Lab Breast Canc Res, Dept Breast Canc Pathol & Res Lab, Tianjin 300060, Peoples R China

Recommended Citation:
Tan, Haidong,Fu, Li,Seno, Masaharu. Optimization of Bacterial Plasmid Transformation Using Nanomaterials Based on the Yoshida Effect[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2010,11(12):4962-4973.
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