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题名: Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells
作者: Ye, Nannan1;  Wang, Ming-Wei2;  Qin, Jianhua1;  Lin, Bingcheng1
关键词: Microfluidic ;  Formyl peptide receptor ;  Chemotaxis ;  Calcium mobilization ;  FPR ligand uptake
刊名: BIOMEDICAL MICRODEVICES
发表日期: 2010-06-01
DOI: 10.1007/s10544-010-9408-3
卷: 12, 期:3, 页:513-521
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Nanoscience & Nanotechnology
研究领域[WOS]: Engineering ;  Science & Technology - Other Topics
英文摘要: The human formyl peptide receptor (FPR) plays an important role in inflammation and immunity. Finding of specific agonists and antagonists of FPR may provide potential therapeutic agents for FPR related disorders. The binding of agonist by FPR induces a cascade of G protein-mediated signaling events leading to neutrophil chemotaxis, intracellualr calcium mobilization, FPR ligand uptake and so on. This work proposed a microfluidic-based method to characterize FPR-related cellular events in response to small peptides, N-formyl-Met-Leu-Phe (fMLF), in rat basophilic leukemia cell line RBL-2H3 expressing human FPR (RBL-FPR). The results showed that fMLF triggered chemotaxis, calcium mobilization and FPR ligand uptake in RBL-FPR cells, indicating the potential role of FPR agonist. The chemotaxis index and the calcium mobilization intensity increased but the time course of calcium mobilization decreased, as the rising of fMLF concentration. The basic agreement between the microfluidic results and the previous studies demonstrated good feasibility of the microfluidic method for characterization of FPR agonist. Microfluidic technology displays significant advantages over traditional methods in terms of sample consumption and assay time. It also facilitates experimental process and real-time observation of cellular responses at single cell resolution.
关键词[WOS]: QUANTUM DOTS ;  CHEMOTACTIC AGONIST ;  TYR-MET ;  GLUTATHIONE ;  FORMYL-PEPTIDE-RECEPTOR-LIKE-1 ;  ACTIVATION ;  EXPRESSION ;  APOPTOSIS ;  BINDING ;  SYSTEM
语种: 英语
WOS记录号: WOS:000277003600016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142126
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Natl Ctr Drug Screening, Shanghai 201203, Peoples R China

Recommended Citation:
Ye, Nannan,Wang, Ming-Wei,Qin, Jianhua,et al. Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells[J]. BIOMEDICAL MICRODEVICES,2010,12(3):513-521.
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