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Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells
Ye, Nannan1; Wang, Ming-Wei2; Qin, Jianhua1; Lin, Bingcheng1
关键词Microfluidic Formyl Peptide Receptor Chemotaxis Calcium Mobilization Fpr Ligand Uptake
刊名BIOMEDICAL MICRODEVICES
2010-06-01
DOI10.1007/s10544-010-9408-3
12期:3页:513-521
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Technology
类目[WOS]Engineering, Biomedical ; Nanoscience & Nanotechnology
研究领域[WOS]Engineering ; Science & Technology - Other Topics
关键词[WOS]QUANTUM DOTS ; CHEMOTACTIC AGONIST ; TYR-MET ; GLUTATHIONE ; FORMYL-PEPTIDE-RECEPTOR-LIKE-1 ; ACTIVATION ; EXPRESSION ; APOPTOSIS ; BINDING ; SYSTEM
英文摘要The human formyl peptide receptor (FPR) plays an important role in inflammation and immunity. Finding of specific agonists and antagonists of FPR may provide potential therapeutic agents for FPR related disorders. The binding of agonist by FPR induces a cascade of G protein-mediated signaling events leading to neutrophil chemotaxis, intracellualr calcium mobilization, FPR ligand uptake and so on. This work proposed a microfluidic-based method to characterize FPR-related cellular events in response to small peptides, N-formyl-Met-Leu-Phe (fMLF), in rat basophilic leukemia cell line RBL-2H3 expressing human FPR (RBL-FPR). The results showed that fMLF triggered chemotaxis, calcium mobilization and FPR ligand uptake in RBL-FPR cells, indicating the potential role of FPR agonist. The chemotaxis index and the calcium mobilization intensity increased but the time course of calcium mobilization decreased, as the rising of fMLF concentration. The basic agreement between the microfluidic results and the previous studies demonstrated good feasibility of the microfluidic method for characterization of FPR agonist. Microfluidic technology displays significant advantages over traditional methods in terms of sample consumption and assay time. It also facilitates experimental process and real-time observation of cellular responses at single cell resolution.
语种英语
WOS记录号WOS:000277003600016
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142126
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116023, Peoples R China
2.Natl Ctr Drug Screening, Shanghai 201203, Peoples R China
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GB/T 7714
Ye, Nannan,Wang, Ming-Wei,Qin, Jianhua,et al. Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells[J]. BIOMEDICAL MICRODEVICES,2010,12(3):513-521.
APA Ye, Nannan,Wang, Ming-Wei,Qin, Jianhua,&Lin, Bingcheng.(2010).Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells.BIOMEDICAL MICRODEVICES,12(3),513-521.
MLA Ye, Nannan,et al."Microfluidic devices for characterizing the agonist of formyl peptide receptor in RBL-FPR cells".BIOMEDICAL MICRODEVICES 12.3(2010):513-521.
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