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题名: Orthogonal separation on one beta-cyclodextrin column by switching reversed-phase liquid chromatography and hydrophilic interaction chromatography
作者: Feng, Jia-tao1;  Guo, Zhi-mou1;  Shi, Hui2;  Gu, Jiang-ping2;  Jin, Yu2;  Liang, Xin-miao1, 2
关键词: Beta-cyclodextrin bonded stationary phase ;  Dual retention mechanism ;  Hydrophilic interaction chromatography ;  Reversed-phase liquid chromatography ;  Natural product
刊名: TALANTA
发表日期: 2010-06-15
DOI: 10.1016/j.talanta.2010.03.007
卷: 81, 期:4-5, 页:1870-1876
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A dual retention combined with reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) has been observed on beta-cyclodextrin (beta-CD) bonded stationary phase. A typical U-shaped retention curve was achieved owing to dual retention mechanism Based on this observation, a beta-CD column can be operated under reversed-phase liquid chromatography (RP-LC) and hydrophilic interaction chromatography (HILIC) modes. Two-dimensional liquid chromatography (2D-LC) analysis can be realized on Just a beta-CD column by switching these two different separation modes. In this study, off-line 2D-LC analysis for a natural product was carried out to prove the orthogonal separation between RP-LC and HILIC modes on a Click beta-CD column. Herba Hedyotis Diffusae, the whole grass of Hedyotis Diffusae wild was extracted with water, pretreated with macroporous resin and then first separated at RP-LC mode on the Click beta-CD column to obtain successive fractions, which were then reanalyzed at HILIC mode on the same Click beta-CD column The result proved that both separation modes on the Click beta-CD column have good retention and peak shape, and these two separation modes have good orthogonality 2D-LC analysis revealed abundant information in the natural product. Especially numerous minor components were enriched and separated. The mobile phase used in RP-LC and HILIC modes can be same and the switch between these two separation modes is easily realized by changing the ratio of the acetonitrile and water. Hence the mobile phase in this 2D-LC system is completely compatible. This advantage makes this combination is an appropriate 2D-LC method for the solutes having retention at both separation modes (C) 2010 Elsevier B V All rights reserved
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  HEDYOTIS-DIFFUSA ;  IRIDOID GLUCOSIDES ;  POLAR COMPOUNDS ;  SILICA COLUMNS ;  BONDED PHASE ;  HILIC-RP ;  IDENTIFICATION ;  PEPTIDES ;  SYSTEM
语种: 英语
WOS记录号: WOS:000278737100113
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142177
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China

Recommended Citation:
Feng, Jia-tao,Guo, Zhi-mou,Shi, Hui,et al. Orthogonal separation on one beta-cyclodextrin column by switching reversed-phase liquid chromatography and hydrophilic interaction chromatography[J]. TALANTA,2010,81(4-5):1870-1876.
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