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题名: Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles
作者: Chen, Qiang1;  Tang, Wei1;  Wang, Dingzhong1;  Wu, Xiaojie1;  Li, Na1;  Liu, Feng1
关键词: Aptamer ;  Gold nanoparticles ;  Amplification ;  QCM-D biosensor ;  Human alpha-thrombin
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2010-10-15
DOI: 10.1016/j.bios.2010.07.034
卷: 26, 期:2, 页:575-579
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: A highly sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor for protein was developed using aptamer-functionalized gold nanoparticles (Apt-GNPs) for amplification. Human alpha-thrombin, an important physiological protease found in blood, was chosen as the target protein. Captured by immobilized aptamers, thrombin was determined on-line using Apt-GNPs to enhance both frequency and dissipation signals. The fabricated sandwich of aptamer/thrombin/Apt-GNPs on chip surface was confirmed by atomic force microscopy (AFM). Compared to direct assay, the detection limit for thrombin was down to 0.1 nM, yielding about 2 orders of magnitude improvement in sensitivity. This aptamer-based QCM-D biosensor also showed good selectivity and repeatability in complex matrix. For the first time, the dual-signal enhancement of Apt-GNPs on QCM-D sensing was demonstrated, and such design could provide a promising detection strategy for proteins with two binding sites. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: HUMAN THROMBIN ;  DNA ;  LIGANDS ;  BIND ;  RNA ;  MOLECULES ;  SELECTION ;  SENSORS ;  SIGNAL
语种: 英语
WOS记录号: WOS:000283804400043
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142207
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China

Recommended Citation:
Chen, Qiang,Tang, Wei,Wang, Dingzhong,et al. Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles[J]. BIOSENSORS & BIOELECTRONICS,2010,26(2):575-579.
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