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Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles
Chen, Qiang; Tang, Wei; Wang, Dingzhong; Wu, Xiaojie; Li, Na; Liu, Feng
关键词Aptamer Gold Nanoparticles Amplification Qcm-d Biosensor Human Alpha-thrombin
刊名BIOSENSORS & BIOELECTRONICS
2010-10-15
DOI10.1016/j.bios.2010.07.034
26期:2页:575-579
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]HUMAN THROMBIN ; DNA ; LIGANDS ; BIND ; RNA ; MOLECULES ; SELECTION ; SENSORS ; SIGNAL
英文摘要A highly sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) biosensor for protein was developed using aptamer-functionalized gold nanoparticles (Apt-GNPs) for amplification. Human alpha-thrombin, an important physiological protease found in blood, was chosen as the target protein. Captured by immobilized aptamers, thrombin was determined on-line using Apt-GNPs to enhance both frequency and dissipation signals. The fabricated sandwich of aptamer/thrombin/Apt-GNPs on chip surface was confirmed by atomic force microscopy (AFM). Compared to direct assay, the detection limit for thrombin was down to 0.1 nM, yielding about 2 orders of magnitude improvement in sensitivity. This aptamer-based QCM-D biosensor also showed good selectivity and repeatability in complex matrix. For the first time, the dual-signal enhancement of Apt-GNPs on QCM-D sensing was demonstrated, and such design could provide a promising detection strategy for proteins with two binding sites. (C) 2010 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000283804400043
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142207
专题中国科学院大连化学物理研究所
作者单位Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
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GB/T 7714
Chen, Qiang,Tang, Wei,Wang, Dingzhong,et al. Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles[J]. BIOSENSORS & BIOELECTRONICS,2010,26(2):575-579.
APA Chen, Qiang,Tang, Wei,Wang, Dingzhong,Wu, Xiaojie,Li, Na,&Liu, Feng.(2010).Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles.BIOSENSORS & BIOELECTRONICS,26(2),575-579.
MLA Chen, Qiang,et al."Amplified QCM-D biosensor for protein based on aptamer-functionalized gold nanoparticles".BIOSENSORS & BIOELECTRONICS 26.2(2010):575-579.
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