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Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta
Wang, Wei; Han, Guanghui2; Ye, Mingliang2; Shi, Huili; Zou, Hanfa2; Huo, Keke1
关键词Mrk Beta Msk1 Multiple Stage Ms Phosphorylation
刊名ELECTROPHORESIS
2010-04-01
DOI10.1002/elps.200900637
31期:8页:1283-1293
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]PROTEIN-INTERACTION NETWORK ; INDIVIDUAL PHOSPHOPROTEINS ; PHOSPHOPEPTIDE ENRICHMENT ; CREB ; KINASE ; FAMILY ; ERK ; CHROMATOGRAPHY ; SEARCH ; MAPK
英文摘要The desire to map reliable phosphorylation signaling network has motivated the development of high-performance techniques. Targeted biochemical studies and updated methods employing MS techniques are most used in mapping the phosphorylation sites and verifying novel interactions of kinases. Previously, we have established a novel method to efficiently facilitate more comprehensive, accurate phosphorylation site mapping of individual phosphoproteins by using combination of multiple stage MS analysis with target-decoy database search against the much smaller targeted database. In this study, by applying this method, we have identified the phosphorylation sites in human MSK1 mitogen- and stress-activated protein kinase 1), which has been proved to be a multi-phosphorylated kinase that plays key roles in various cell functions, activated by a novel interaction with MRK-beta. The results show that this method can find out not only those previously identified active sites in MSK1, but also some novel phosphorylated sites, which correlates with biochemical evidence that, besides p38 and extracellular signal-regulated kinase, MRK-beta could also activate MSK1 through direct interaction. Hence, we conclude this method is sensitive and reliable as expected and it can be further combined with automated screening and biochemical study in efficiently building up a more comprehensive phosphoprotein network.
语种英语
WOS记录号WOS:000277778900001
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142225
专题中国科学院大连化学物理研究所
作者单位1.Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China
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GB/T 7714
Wang, Wei,Han, Guanghui,Ye, Mingliang,et al. Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta[J]. ELECTROPHORESIS,2010,31(8):1283-1293.
APA Wang, Wei,Han, Guanghui,Ye, Mingliang,Shi, Huili,Zou, Hanfa,&Huo, Keke.(2010).Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta.ELECTROPHORESIS,31(8),1283-1293.
MLA Wang, Wei,et al."Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta".ELECTROPHORESIS 31.8(2010):1283-1293.
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