DICP OpenIR
Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta
Wang, Wei; Han, Guanghui2; Ye, Mingliang2; Shi, Huili; Zou, Hanfa2; Huo, Keke1
KeywordMrk Beta Msk1 Multiple Stage Ms Phosphorylation
Source PublicationELECTROPHORESIS
2010-04-01
DOI10.1002/elps.200900637
Volume31Issue:8Pages:1283-1293
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordPROTEIN-INTERACTION NETWORK ; INDIVIDUAL PHOSPHOPROTEINS ; PHOSPHOPEPTIDE ENRICHMENT ; CREB ; KINASE ; FAMILY ; ERK ; CHROMATOGRAPHY ; SEARCH ; MAPK
AbstractThe desire to map reliable phosphorylation signaling network has motivated the development of high-performance techniques. Targeted biochemical studies and updated methods employing MS techniques are most used in mapping the phosphorylation sites and verifying novel interactions of kinases. Previously, we have established a novel method to efficiently facilitate more comprehensive, accurate phosphorylation site mapping of individual phosphoproteins by using combination of multiple stage MS analysis with target-decoy database search against the much smaller targeted database. In this study, by applying this method, we have identified the phosphorylation sites in human MSK1 mitogen- and stress-activated protein kinase 1), which has been proved to be a multi-phosphorylated kinase that plays key roles in various cell functions, activated by a novel interaction with MRK-beta. The results show that this method can find out not only those previously identified active sites in MSK1, but also some novel phosphorylated sites, which correlates with biochemical evidence that, besides p38 and extracellular signal-regulated kinase, MRK-beta could also activate MSK1 through direct interaction. Hence, we conclude this method is sensitive and reliable as expected and it can be further combined with automated screening and biochemical study in efficiently building up a more comprehensive phosphoprotein network.
Language英语
WOS IDWOS:000277778900001
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142225
Collection中国科学院大连化学物理研究所
Affiliation1.Fudan Univ, State Key Lab Genet Engn, Inst Genet, Sch Life Sci, Shanghai 200433, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Wang, Wei,Han, Guanghui,Ye, Mingliang,et al. Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta[J]. ELECTROPHORESIS,2010,31(8):1283-1293.
APA Wang, Wei,Han, Guanghui,Ye, Mingliang,Shi, Huili,Zou, Hanfa,&Huo, Keke.(2010).Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta.ELECTROPHORESIS,31(8),1283-1293.
MLA Wang, Wei,et al."Mapping of phosphorylation sites in human MSK1 activated by a novel interaction with MRK-beta".ELECTROPHORESIS 31.8(2010):1283-1293.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Wei]'s Articles
[Han, Guanghui]'s Articles
[Ye, Mingliang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Wei]'s Articles
[Han, Guanghui]'s Articles
[Ye, Mingliang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Wei]'s Articles
[Han, Guanghui]'s Articles
[Ye, Mingliang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.