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题名: The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA
作者: Gai, Qingqing1;  Qu, Feng1;  Zhang, Yukui2
关键词: bovine hemoglobin ;  bovine serum albumin ;  molecularly imprinted gel polymer
刊名: SEPARATION SCIENCE AND TECHNOLOGY
发表日期: 2010
DOI: 10.1080/01496395.2010.484409
卷: 45, 期:16, 页:2394-2399
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Multidisciplinary ;  Engineering, Chemical
研究领域[WOS]: Chemistry ;  Engineering
英文摘要: Molecularly imprinted gel polymer (MIP) for the selective imprinting of bovine hemoglobin (BHb) was prepared in aqueous media by bulk polymerization using polyacrylamide matrix. The synthesis conditions of BHb-MIP were investigated, which involved the interaction of functional monomers and template protein in different molar ratio, solution pH, and ionic strength. The adsorption experiments indicated that BHb-MIP had a high affinity for BHb over the non-imprinted polymers. The selectivity of BHb-MIP for BHb and bovine serum albumin (BSA) with similar molecular weight was compared. It was demonstrated that BHb-MIP had better selective adsorption and recognition properties to BHb especially in the presence of BSA as competing protein. It might be helpful for selectively separating template protein with MIP from the proteins mixture with similar molecular weight.
关键词[WOS]: ELECTROSTATIC FUNCTIONAL-GROUPS ;  SOLID-PHASE EXTRACTION ;  BOVINE SERUM-ALBUMIN ;  SUBMICROMETER PARTICLES ;  PROTEIN RECOGNITION ;  CHITOSAN BEADS ;  SEPARATION ;  POLYMERIZATION ;  CHROMATOGRAPHY ;  HEMOGLOBIN
语种: 英语
WOS记录号: WOS:000284768500017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142229
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci & Technol, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Gai, Qingqing,Qu, Feng,Zhang, Yukui. The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA[J]. SEPARATION SCIENCE AND TECHNOLOGY,2010,45(16):2394-2399.
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