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Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay
Xiao, Jing1,2; Zhang, Ying1; Wang, Jianzheng1,2; Yu, Weiting1; Wang, Wei1; Ma, Xiaojun1
关键词Microencapsulated Cell Apa Resazurin Mtt Non-invasive Monitoring Of Cell Proliferation
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
2010-11-01
DOI10.1007/s12010-010-8975-3
162期:7页:1996-2007
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]MESENCHYMAL STEM-CELLS ; IN-VITRO ; ALAMARBLUE ASSAY ; BIOLUMINESCENCE ; METABOLISM ; CULTURES ; GROWTH ; TUMOR ; MODEL ; BLUE
英文摘要Cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy and large-scale cell culture. For better quality control, it is important to accurately measure the microencapsulated cell viability and proliferation in the culture. A number of assays have been used for this purpose, but limitations arise. In this study, we investigated the feasibility and reliability of resazurin as a cell growth indicator in microencapsulated culture system. According to the experiment data, there was a reversible, time- and dose-dependent growth inhibition as observed for resazurin application in encapsulated cells. A positive relationship was observed between reduction of resazurin and CHO cell number in microcapsule. Moreover, the resazurin assay provided an equivalent result to the commonly used MTT method in determining CHO cell proliferation in APA microcapsule with no notable influence on cell distribution and organization pattern. In conclusion, resazurin assay is offered as a simple, rapid and non-invasive method for in vitro microencapsulated cell viability and proliferation measurement.
语种英语
WOS记录号WOS:000282315100015
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142231
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Lab Biomed Mat Engn, Dept Sci & Technol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714
Xiao, Jing,Zhang, Ying,Wang, Jianzheng,et al. Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2010,162(7):1996-2007.
APA Xiao, Jing,Zhang, Ying,Wang, Jianzheng,Yu, Weiting,Wang, Wei,&Ma, Xiaojun.(2010).Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,162(7),1996-2007.
MLA Xiao, Jing,et al."Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 162.7(2010):1996-2007.
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