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题名: Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay
作者: Xiao, Jing1, 2;  Zhang, Ying1;  Wang, Jianzheng1, 2;  Yu, Weiting1;  Wang, Wei1;  Ma, Xiaojun1
关键词: Microencapsulated cell ;  APA ;  Resazurin ;  MTT ;  Non-invasive monitoring of cell proliferation
刊名: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
发表日期: 2010-11-01
DOI: 10.1007/s12010-010-8975-3
卷: 162, 期:7, 页:1996-2007
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Biotechnology & Applied Microbiology
英文摘要: Cell microencapsulation is a promising approach for cell implantation, cell-based gene therapy and large-scale cell culture. For better quality control, it is important to accurately measure the microencapsulated cell viability and proliferation in the culture. A number of assays have been used for this purpose, but limitations arise. In this study, we investigated the feasibility and reliability of resazurin as a cell growth indicator in microencapsulated culture system. According to the experiment data, there was a reversible, time- and dose-dependent growth inhibition as observed for resazurin application in encapsulated cells. A positive relationship was observed between reduction of resazurin and CHO cell number in microcapsule. Moreover, the resazurin assay provided an equivalent result to the commonly used MTT method in determining CHO cell proliferation in APA microcapsule with no notable influence on cell distribution and organization pattern. In conclusion, resazurin assay is offered as a simple, rapid and non-invasive method for in vitro microencapsulated cell viability and proliferation measurement.
关键词[WOS]: MESENCHYMAL STEM-CELLS ;  IN-VITRO ;  ALAMARBLUE ASSAY ;  BIOLUMINESCENCE ;  METABOLISM ;  CULTURES ;  GROWTH ;  TUMOR ;  MODEL ;  BLUE
语种: 英语
WOS记录号: WOS:000282315100015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142231
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Lab Biomed Mat Engn, Dept Sci & Technol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Xiao, Jing,Zhang, Ying,Wang, Jianzheng,et al. Monitoring of Cell Viability and Proliferation in Hydrogel-Encapsulated System by Resazurin Assay[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2010,162(7):1996-2007.
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