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Protein separation on a polar-copolymerized C-8 stationary phase
Dong, Jun; Yu, Long; Zhang, Xiuli; Xue, Xingya; Guo, Zhimou; Liang, Xinmiao
KeywordReversed-phase Liquid Chromatography Polar-copolymerized Protein Separation Glycoprotein
Source PublicationANALYTICAL AND BIOANALYTICAL CHEMISTRY
2011-04-01
DOI10.1007/s00216-010-4462-5
Volume399Issue:10Pages:3415-3421
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHYDROPHOBIC INTERACTION CHROMATOGRAPHY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; ION-EXCHANGE CHROMATOGRAPHY ; LOW PH STABILITY ; AFFINITY-CHROMATOGRAPHY ; SILICA ; GLYCOPROTEINS ; PROTEOMICS ; SYSTEMS
AbstractA novel polar-copolymerized C-8 stationary phase composed of octyl and chloropropyl (C-8-C3Cl)-bonded silica has been developed for protein separation. Separation of standard proteins on this homemade column was investigated and compared with separation on a Waters Symmetry 300 C-4 column. Results indicated that the chromatographic performance of the homemade column in the separation of proteins was excellent. The new polar copolymerized C-8 stationary phase enabled glycoprotein separation with good resolution and reproducibility. It was also used for purification of ovalbumin glycoprotein in order to improve identification of the protein.
Language英语
WOS IDWOS:000288398700011
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142312
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Dong, Jun,Yu, Long,Zhang, Xiuli,et al. Protein separation on a polar-copolymerized C-8 stationary phase[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3415-3421.
APA Dong, Jun,Yu, Long,Zhang, Xiuli,Xue, Xingya,Guo, Zhimou,&Liang, Xinmiao.(2011).Protein separation on a polar-copolymerized C-8 stationary phase.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,399(10),3415-3421.
MLA Dong, Jun,et al."Protein separation on a polar-copolymerized C-8 stationary phase".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 399.10(2011):3415-3421.
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