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题名: Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry
作者: Dong Jun1;  Cai Xiao-ming1;  Zou Li-juan2;  Chen Cheng1;  Xue Xing-ya1;  Zhang Xiu-li1;  Liang Xin-miao1
关键词: Metabonomics ;  Sample preparation ;  Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF MS) ;  Lung cancer ;  Biomarker
刊名: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
发表日期: 2011-09-01
卷: 27, 期:5, 页:750-755
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: Centrifugal ultrafiltration after methanol extraction of whole plasma was used as an optimal condition for the preparation of blood plasma before metabonomic studies. The plasma samples from 102 lung cancer patients and 34 healthy volunteers were prepared with this approach. With ultra-performance liquid chromatography(UPLC) coupled with quadrupole time-of-flight mass spectrometry(Q-TOF MS) analysis, the samples were investigated in order to find potential disease biomarkers. After data acquisition, orthogonal signal correction partial least squares models were built to differentiate the healthy volunteers from lung cancer patients and to identify metabolites that showed significantly different expression between the two groups. Several metabolite ions were identified as potential biomarkers according to the variable importance in the project(VIP) value in both ion modes. Five lysophosphatidylcholines were further identified as specifically lysoPC 16:0, isomer of lysoPC 16:0, lysoPC 18:0, lysoPC 18:1 and lysoPC 18:2. These results suggest that UPLC coupled with Q-TOF MS is an effective technique for the analysis of plasma metabolites in metabonomic studies.
关键词[WOS]: EARLY-DIAGNOSIS ;  STRUCTURAL DETERMINATION ;  SAMPLE PREPARATION ;  HUMAN PLASMA ;  UPLC-MS ;  METABONOMICS ;  SERUM ;  PRECIPITATION ;  METABOLITES ;  STATISTICS
语种: 英语
WOS记录号: WOS:000296120800009
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142320
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116044, Peoples R China

Recommended Citation:
Dong Jun,Cai Xiao-ming,Zou Li-juan,et al. Lysophosphatidylcholine Biomarkers of Lung Cancer Detected by Ultra-performance Liquid Chromatography Coupled with Quadrupole Time-of-flight Mass Spectrometry[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(5):750-755.
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