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题名: In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation
作者: Sun, Xiangli1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
关键词: Click chemistry ;  High-performance liquid chromatography ;  Polymer monolithic column ;  Protein separation
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-04-01
DOI: 10.1007/s00216-010-4390-4
卷: 399, 期:10, 页:3407-3413
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Two types of macroporous organic polymer monoliths based on glycidyl methacrylate (GMA), 4-vinylbenzyl chloride (VBC) and divinylbenzene (DVB) were prepared inside stainless-steel tubes. Azide functionalities were firstly introduced on the surfaces of poly(GMA-co-DVB) and poly(VBC-co-DVB) monoliths to provide reactive sites for click chemistry. With the application of copper(I)-catalyzed (3 + 2) azide-alkyne cycloaddition, an in-column click-modification approach for covalent attachment of long alkyl chains onto polymer monoliths was developed. The column morphology and surface chemistry of the fabricated monolithic columns were characterized by the scanning electron microscopy, mercury intrusion porosimeter, Fourier transform infrared spectroscopy, and elemental analyses, respectively. The chromatographic performances of the "clicked" stationary phases were demonstrated with the high separation efficiency for a variety of proteins within 4 min.
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ;  RIGID POLYMER MONOLITHS ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  EFFICIENT STRATEGY ;  TERMINAL ALKYNES ;  CHEMISTRY ;  MEDIA ;  SUPPORTS ;  FACILE ;  AZIDES
语种: 英语
WOS记录号: WOS:000288398700010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142323
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Sun, Xiangli,He, Xiwen,Chen, Langxing,et al. In-column "click" preparation of hydrophobic organic monolithic stationary phases for protein separation[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(10):3407-3413.
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